IV ... MYCOTAXON 132(3)

MYCOTAXON

VOLUME ONE HUNDRED THIRTY-TWO (3) — TABLE OF CONTENTS

132-3: TABLE OF CONTENTS, NOMENCLATURAL UPDATES, PEERS & EDITORIALS

Nomenclatural novelties & typifications proposed in 132(3)............. vii

TROVICWCES mM le ASh at ov ans Wile cine Mins vam MON Re, eerie | walle ee ix

DOLZ SHOES OND I OCOG ANE oo 35 co Pamcs ORE tg Macias ee ads Ok mes earns oe eh aE x

EVOILITE EQ IEOl™ Pic rain Bbcg card cetera, task PoE Lreshh cine ba Marat ee eM rg xi

TER CUETO ATIC Mal oh od hPa ag Pic cachet: Peed Dig sea setheh itch hehe eee iss xii

RESEARCH ARTICLES

Lactifluus persicinus sp. nov. from the gallery forests

of West Cameroon Lynn DELGAT, EskE DE Crop,

ANDRE-LEDOUX NJOUONKOU & ANNEMIEKE VERBEKEN 471

Distoceratosporella digitiformis gen. & sp. nov. from Brazil,

Alcornia sessilispora gen. & comb. nov., and three new

Distoceratosporella combinations JOSIANE SANTANA MONTEIRO,

SHEILA MIRANDA LEAO-FERREIRA, LUIS FERNANDO PASCHOLATI GUSMAO,

PauL M. Kirk & RAFAEL F, CasTANEDA-Ruiz 483

First report of Colletotrichum fioriniae infection of hazelnut

ARZU SEZER, FATMA SARA DOLAR & Fitz Unat 495

New Calbovista, Mycena, Rhizopogon, Stictis, and

Symphyosirinia records from Turkey IBRAHIM TURKEKUL 503

Tripospermum sinense sp. nov. from China Min Qiao, Yinc HuANG,

Cuu DENG & ZE-FEN Yu 513

Calceispora hachijoensis and Paratomenticola lanceolata,

two new records for China X1a0-MEI Wana, Guo-NIn Liv,

X1a0-MaN Liu & X1A0-FENG Du 519

Records of Beltrania rhombica and Didymobotryum rigidum

from China X1a0-MEI Wana, Guo-Nn Liv,

SHAN-SHAN CHEN & XIAO-FENG Du 525

New records of Lepraria and Trapelia from China

SHU-KUN YAN, XIANG-XIANG ZHAO, ZHAO-JIE REN & LU-LU ZHANG 531

Lecanora subloekoesii sp. nov. and four other species

of the L. subfusca group new to China Ler LU & ZUN-TIAN ZHAO 539

Neosporidesmium wuyishanense sp. nov. from southern China,

and a first Chinese record of Morrisographium ulmi

Hao-Hua LI, Kat ZHANG, JI-WEN XIA,

CHUN-LING YANG, JIN-YE WANG & XIU-GUO ZHANG 547

JULY-SEPTEMBER 2017... V

Notes on Vamsapriya and V. camagueyensis comb. nov.

RAFAEL F. CASTANEDA-RUuIz, XIU-GUO ZHANG,

DeE-WEI Li, Luis FERNANDO PASCHOLATI GUSMAO,

SIMON PEREZ-MARTINEZ & DAYNET Sosa 553

Wiesneriomyces machilicola sp. nov. from China

DE-WEI LI, JING- YUAN CHEN & YI-XUN WANG 559

Gonatophragmiopsis verrucosa gen. & sp. nov.

and Pithomyces dimorphosporus sp. nov. from Brazil

Luis FERNANDO PASCHOLATI GUSMAO,

JOSIANE SANTANA MONTEIRO & RAFAEL F. CASTANEDA-RuIz 565

Eight new records of lichenized and lichenicolous fungi

for Turkey MEHMET CANDAN 575

Zygosporium verruciferum sp. nov., on Solanum lycopersicum

from Venezuela LILIANYEL LUCENA & ROBERTO C. FERNANDEZ VALENCIA 585

Diaporthe camptothecicola sp. nov., on Camptotheca acuminata

in China QIN YANG, XIN-LEI FAN,

ZHUO Du, YING-MEI LIANG & CHENG-MING TIAN 591

New species of Cephalotrichum, Leptographium, and

Myrothecium from soil in China Yu-LAN JIANG, YUE-MING Wu,

BIN YANG, JUN-JIE XU, ZHENG-GAO ZHANG, YUE-LI ZHANG & TIAN-YU ZHANG 603

First record of bioluminescence in fungi from Mexico

ALONSO CoRTES PEREZ, FLORENCIA RAMIREZ GUILLEN,

ROSARIO MEDEL & ALAN ROCKEFELLER 611

Catenularia variegata sp. nov. from southern China

and a first Chinese record of Xylocladium clautriavii

Hao-Hua LI, Kal ZHANG, JI-WEN XIA,

JIN-YE WANG, CHUN-LING YANG & XIU-GUO ZHANG 621

Drechslera, Fusariella, Coniochaeta, and Pyricularia spp. nov.

from soil in China Yu-LAN JIANG, YUE-MING Wu, ZHENG-GAO ZHANG,

Jin-Hua Kono, HONG-FENG WANG & TIAN-YU ZHANG 627

Tuber magnatum in Thailand, a first report from Asia

NAKARIN SUWANNARACH, JATURONG KUMLA,

JOMKWAN MEERAK & SAISAMORN LUMYONG 635

Craspedodidymella matogrossensis gen. & sp. nov.

from the Brazilian Amazon rainforest FLAVIA RODRIGUES BARBOSA,

Luis FERNANDO PASCHOLATI GUSMAO & RAFAEL FELIPE CASTANEDA-RuIz 643

Notes on some myxomycetes from Crimea (Ukraine)

G. MorENO, A. LOPEZ-VILLALBA, A. CASTILLO,

K.O. ROMANENKO & D.V. LEONTYEV 649

VI ... MYCOTAXON 132(3)

Amanita alpinicola sp. nov., associated with Pinus albicaulis,

a western 5-needle pine

CaTuy L. Cripps, JANET E. LINDGREN & EDWARD G. BARGE 665

Cladosporium, Phialophora, Pseudoramichloridium &

Ticogloea spp. nov. from China Yu-Lan Jiane, YuE-Minc Wu, BIN YANG,

JUN-JIE Xu, ZHENG-GAO ZHANG, JIN-Hua KONG & TIAN-YU ZHANG 677

Phylloporus brunneiceps from Pakistan

A. NASEER, A.N. KHALID & A.R. Nrazi 685

Zelotetraploa aquatica gen. & sp. nov. and Blastoheterospora catenata

gen. & sp. nov., from submerged wood from Brazil

JOSIANE SANTANA MONTEIRO,

Luis FERNANDO PasCHOLATI GUSMAO & RAFAEL F. CASTANEDA-RuIz 695

REGIONAL MYCOBIOTA NEW TO THE MYCOTAXON WEBSITE

Fungi and fungus-like organisms of Homilsha Forests National Park, Ukraine 705

OLEH V. PRYLUTSKYI, OLEXANDER YU. AKULOV, Dmitry V. LEONTYEV,

ALEXANDER V. ORDYNETS, [RYNA I. YATSIUK, ANDRIY S. USICHENKO &

ANTON O. SAVCHENKO

PUBLICATION DATE FOR VOLUME ONE HUNDRED THIRTY-TWO (2)

MYCOTAXON for APRIL-JUNE 2017, (I-xx + 243-470)

was issued on July 12, 2017

JULY-SEPTEMBER 2017... VII

NOMENCLATURAL NOVELTIES AND TYPIFICATIONS

PROPOSED IN MYCOTAXON 132(3)

Alcornia J.S. Monteiro, S.M. Ledo, Gusmao, P.M. Kirk & R.F. Castafieda

[IF 553580], p. 486

Alcornia sessilispora (Alcorn & P.M. Kirk) J.S. Monteiro, $.M. Leao, Gusmao,

PM. Kirk & R.F. Castafieda [IF553581], p. 486

Amanita alpinicola C. Cripps & J. Lindgr.

[MB 812991], p. 669

Blastoheterospora J.S. Monteiro, Gusmao & R.E. Castaneda

[MB 822124], p. 698

Blastoheterospora catenata J.S. Monteiro, Gusmao & R.F. Castafieda

[MB 822125], p. 698

Catenularia variegata H.H. Li & X.G. Zhang

[MB 822506], p. 621

Cephalotrichum turriforme T.Y. Zhang, Y.L. Jiang & Y.M. Wu

[MB 820187], p. 604

Cladosporium ningboense J.J. Xu, Y.M. Wu & T.Y. Zhang

[MB 820628], p. 678

Coniochaeta xinjiangensis J.H. Kong & TY. Zhang

[MB 822522], p. 630

Craspedodidymella E.R. Barbosa, R.F. Castafieda & Gusmao

[MB 820502], p. 644

Craspedodidymella mattogrossensis ER. Barbosa, R.F. Castafeda & Gusmao

[MB 820503], p. 644

Diaporthe camptothecicola C.M. Tian & Qin Yang

[MB 822351], p. 596

Distoceratosporella J.S. Monteiro, Gusmao, & R.F. Castafieda

[IF 553582], p. 489

Distoceratosporella amazonica (J.S. Monteiro & Gusmao) J.S. Monteiro,

Gusmao, & R.E. Castafeda [IF 553584], p. 492

Distoceratosporella digitiformis J.S. Monteiro, Gusmao & R.F. Castaneda

[IF 553583], p. 490

Distoceratosporella hernandezii (J.S. Monteiro & Gusmao) J.S. Monteiro,

Gusmao & R.F. Castafieda [IF 553585], p. 492

Distoceratosporella longiramosa (R.F. Castafieda) R.F. Castaneda,

J.S. Monteiro & Gusmao [IF 553586], p. 492

Drechslera elliptica H.F. Wang & T.Y. Zhang

[MB 819404], p. 627

Fusariella verrucosa J.H. Kong & T.Y. Zhang

[MB 819405], p. 630

vill ... MYCOTAXON 132(3)

Gonatophragmiopsis Gusmao, J.S. Monteiro, & R.E. Castafieda

[MB 822036], p. 566

Gonatophragmiopsis verrucosa Gusmao, J.S. Monteiro, & R.F. Castaneda

[MB 822037], p. 566

Lactifluus persicinus Delgat & De Crop

[MB 817873], p. 475

Lecanora subloekoesii Z.T. Zhao & L. Li

[MB 812188], p. 540

Leptographium lateritium J.J. Xu & T.Y. Zhang

[MB 818979], p. 606

Myrothecium curvisporum Y.L. Zhang & TY. Zhang

[MB 818980]. P. 607

Neosporidesmium wuyishanense H.H. Li & X.G. Zhang

[MB 822164], p. 548

Phialophora dimorphospora J.J. Xu & TY. Zhang

[MB 820631], p. 680

Pithomyces dimorphosporus Gusmao, J.S. Monteiro, & R.F. Castaneda

[MB 822038], p. 569

Pseudoramichloridium xinjiangense T.Y. Zhang & J.H. Kong

[MB 820632], p. 680

Pyricularia korlaensis J.H. Kong & T.Y. Zhang

[MB 820186], p. 631

Ticogloea elliptica T.Y. Zhang, Y.M. Wu & J.J. Xu

[MB 820633], p. 682

Tripospermum sinense Ying Huang & Z.F. Yu

[MB 815346], p. 514

Vamsapriya camagueyensis (R.F. Castafieda) R.F. Castaneda, X.G. Zhang &

Gusmao [IF 552810], p. 554

Wiesneriomyces machilicola D.W. Li, Jing Y. Chen & Yi X. Wang

[MB 819827], p. 560

Zelotetraploa J.S. Monteiro, Gusmao & R.E. Castaneda

[MB 822122], p. 696

Zelotetraploa aquatica J.S. Monteiro, Gusmao & R.F. Castaheda

[MB 822123], p. 696

Zygosporium verruciferum L. Lucena

[MB 821676], p. 586

JULY-SEPTEMBER 2017...

REVIEWERS — VOLUME ONE HUNDRED THIRTY-TWO (3)

The Editors express their appreciation to the following individuals who have,

prior to acceptance for publication, reviewed one or more of the papers

prepared for this quarter.

Najam ul Sehar Afshan

Ilgaz Akata

Michael W. Beug

Wolfgang von Brackel

Irwin M. Brodo

R.E. Castaneda-Ruiz

Dennis E. Desjardin

Ursula Eberhardt

Shouyu Guo

Luis Fernando Pascholati Gusmao

Shah Hussain

Ze-Feng Jia

James C. Lendemer

De-Wei Li

Ying-Ren Lin

Marcos Lizarraga Escobar

Quan Lu

Jian Ma

Eric H.C. McKenzie

Josiane Santana Monteiro

Lorelei L. Norvell

Shaun R. Pennycook

Mykola P. Prydiuk

V. Ramirez-Cruz

Amy Rossman

Irja Saar

Toyozo Sato

Ertugrul Sesli

Steven L. Stephenson

Ave Suija

Wen-Xiu Sun

Andy Taylor

Steven A. Trudell

Else C. Vellinga

Bevan Weir

Jun-Jie Xu

Nian-Kai Zeng

Xiu-Guo Zhang

X ... MYCOTAXON 132(3)

2017 MyYCOTAXON SUBMISSION PROCEDURE

Prospective MycoTaxon authors should download the MycoTaxon 2017 guide, review

& submission forms, and MycoTaxon sample manuscript by clicking the ‘file download

page’ link on our INSTRUCTIONS TO AUTHORS page before preparing their manuscript.

This page summarizes our “4-step’ submission process.

1—PEER REVIEW: Authors first contact two (for journal papers) or three (for

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4—FINAL EDITORIAL REVIEW: Files with errors will be rejected or returned for

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The PDF proof and bibliographic & nomenclatural index entries will be sent

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The Mycotaxon Webmaster <mycotaxon@gmail.com> posts announcements,

subscription & publications information, and author forms & templates on the

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MyYCOTAXON ONLINE— www.ingentaconnect.com/content/mtax/mt

Mycotaxon publishes four quarterly issues per year. Both open access and

subscription articles are offered.

JULY-SEPTEMBER 2017... XI

FROM THE EDITOR-IN-CHIEF

TYPE DESCRIPTIONS AND RANGE EXTENSIONS—In MyCOTAXON 132-1 (ix—x),

we announced our new requirements for papers devoted to range extensions of

previously named taxa. These included the presentation of an original technical

description based on the newly collected specimens, an illustration, a list of

the specimens examined, and a discussion comparing the new record with

previously published descriptions/data for the taxon.

One item we did not cover is the importance of referring to original (type)

descriptions and well-researched monographs to confirm an identification

prior to publishing a new record. Referring to the more general literature

(such as pictorial field guides) are very helpful during the initial identification

process, but it is unwise to rely on literature published for a country well

removed from the new collection: a species reported from England may well

present different features from the type originally described from Japan. The

original type description or a complete description based on material from the

type locality should always be compared with an extralimital collection before

publishing a range extension.

MyYCOTAXON 132(3) contains 27 papers by 114 authors (representing 16

countries) and revised by 38 expert reviewers.

Within its pages are six new genera (Alcornia, Blastoheterospora,

Craspedodidymella, Distoceratosporella, Gonatophragmiopsis, Zelotetraploa,

all from Brazil) and 26 species new to science representing Amanita from

the US.A.; Blastoheterospora, Craspedodidymella, Distoceratosporella,

Gonatophragmiopsis, Pithomyces, Zelotetraploa from Brazil; Catenularia,

Cephalotrichum, Cladosporium, Coniochaeta, Diaporthe, Drechslera, Fusariella,

Lecanora, Leptographium, Myrothecium, Neosporidesmium, Phialophora,

Pseudoramichloridium, Pyricularia, Ticogloea, Tripospermum, Wiesneriomyces

from China; Lactifluus from Cameroon; and Zygosporium from Venezuela.

In addition to range extensions for previously named taxa covering

macrofungi (Pakistan, Thailand, Turkey), hyphomycetes (China), lichens &

lichenicolous fungi (China, Turkey), and myxomycetes (Ukraine), we also offer

new combinations in Alcornia, Distoceratosporella, and Vamsapriya together

with excellent documentations of Colletotrichum fioriniae infecting hazelnut in

Turkey and bioluminescence (Mycena, Panellus) in Mexico.

Warm regards,

Lorelei L. Norvell (Editor-in-Chief)

27 September 2017

XII... MYCOTAXON 132(3)

IN REMEMBRANCE Bt

Marcos FABIO DE OLIVEIRA MARQUES jqiga |

(1980-2017): Marcos Fabio was born on feet

March 28, 1980, in the municipality of Feira =

de Santana, Bahia, Brazil. After graduating in

the biological sciences from State University ©

of Feira de Santana (UEFS), he obtained ~

his masters in Fungal Biology (2001) from ‘

Federal University of Pernambuco (UFPE) _

and his doctorate in Botany (2006) from

UEFS, where he focused on the taxonomy,

ecology, and popularization of mycology

and eventually earned the affectionate

nickname of “Funguinho” from his students

and colleagues. At the time of his sudden

death at the age of 37, Dr. Marques was Full

Professor of the undergraduate Biological

Courtesy of Luis F.P. Gusmao

Sciences program and the Vegetal Biodiversity graduate program and served

as Director of the Department of Education of the State University of Bahia

(DEDC/UNEB - Campus VII).

He concentrated his study on conidial fungi, especially from the

semi-arid region of Northeast of Brazil, where he collected many rare or

uncommon asexual fungi. Marcos had a finely honed sense for new fungal

taxa in the Brazilian Semi-arid region, which he shared with his students.

Listed (as “M.F.O. Marques”) in MycoBank and Index Fungorum as author

or coauthor of two new genera and seven new species, Prof. Marques

contributed to 23 articles on the taxonomy, diversity and ecology of

conidial fungi [https://www.researchgate.net/profile/Marcos_Marques3]. He

published in MycoTaxon (eight papers) and other journals including Acta

BOTANICA BRASILICA, BRAZILIAN JOURNAL OF

BOTANY, JOURNAL OF FORESTRY RESEARCH, and

MYCOLOGIA.

Dr. Luis E P. Gusmao (UEFS), who shared

the above 2015 photograph of his colleague at

the Espago—Ciéncia Micolégica/UNEB, writes:

“Dr. Marcos Fabio Oliveira Marques was one of

ResearchGate photo

my first students in the undergraduate course in

JULY-SEPTEMBER 2017... XIII

biological sciences at UEFS. From the beginning he showed great promise

in mycology. He loved the taxonomy of conidial fungi and soon became a

specialist and master of this group. However, as taxonomy alone was not

enough, he combined ecology with taxonomy for his PhD. I was supervisor

of Marcos in all the phases of his mycological development and I am sure

that we lost a promising and excellent mycologist. My last meeting with

Marcos was in June / 2017 during an evaluation of two doctoral theses of my

students in. Marcos died as a result of a car crash on August 3, 2017”

Danilé Oliveira

MY COTAXON

July-September 2017— Volume 132, pp. 471-483

https://doi.org/10.5248/132.471

Lactifluus persicinus sp. nov.

from the gallery forests of West Cameroon

LYNN DELGAT’, EsKE DE CropP’,

ANDRE-LEDOUX NJOUONKOU? & ANNEMIEKE VERBEKEN'

' Department of Biology, Ghent University,

Karel Lodewijk Ledeganckstraat 35, Ghent, Belgium

? Department of Biological Sciences, University of Bamenda,

39 Bambili, Nord-Ouest, Cameroon

* CORRESPONDENCE TO: lynn.delgat@ugent.be

AsBstRAcT— During field work in the Noun division of western Cameroon during 2011,

2012, and 2014, several collections of an unknown Lactifluus species were discovered in a

gallery forest with Uapaca guineensis. Molecular and morphological research shows that

these collections represent a new species, Lactifluus persicinus, which is described in this

paper. Lactifluus persicinus belongs to Lf sect. Xerampelini in Lf. subg. Pseudogymnocarpi.

Key worps—Russulaceae, ectomycorrhizal fungi, tropical Africa, edible mushroom,

pseudocryptic species

Introduction

The Russulaceae are a family containing important ectomycorrhizal genera.

In 2008, molecular research challenged existing generic concepts, resulting in

the splitting of Russula Pers. and Lactarius Pers. into four segregate genera—

Lactarius, Russula, Multifurca Buyck & V. Hofst., and Lactifluus (Pers.) Roussel

(Buyck et al. 2008). The species-rich milkcap genus Lactifluus (Lf) comprises

about 150 described species worldwide. Furthermore, at least 17 (possibly up to

44) species still await description (De Crop et al. 2017). Lactifluus has a mainly

tropical distribution, with the highest known diversity in tropical Africa (Ba

et al. 2012, De Crop et al. 2012, Maba 2015, Maba et al. 2015a, Van de Putte et

al. 2009, Verbeken & Walleyn 2010) and tropical Asia (Le 2007, Stubbe et al.

472 ... Delgat & al.

TABLE 1. Lactifluus (Lf.) specimens and GenBank sequences used in the molecular

analyses. Type specimens are annotated as [T].

SPECIES

VOUCHER (HERBARIUM)

Lactifluus sect. Pseudogymnocarpi

Lf. cf. pseudogymnocarpus

Lf. cf. pumilus

Lf. gymnocarpoides

Lf. hygrophoroides

Lf. longisporus

Lf. luteopus

Lf. medusae

Lf. pseudoluteopus

Lf. sudanicus

Lactifluus sect. Xerampelini

Lf. persicinus

Uncultured ectomycorrhiza

Lf. goossensiae

Lf. cf. pseudovolemus

Lf. kivuensis

Lf. rubiginosus

Lf. xerampelinu

Lf. sp.

Uncultured ectomycorrhiza

Lf. sp.

Unnamed clade

Lf. sp.

Lf. volemoides

Lactifluus sect. Aurantiifolii

Lf. aurantiifolius

AV 05-085 (GENT)

EDC 12-066 (GENT)

JD 885 (BR)

AV 05-184 (GENT)

AV 05-251 (GENT)

AV 94-557 (GENT) [T]

AV 94-463 (GENT) [T]

EDC 12-152 (GENT)

FH 12-026 (GENT)

AV 11-174 (GENT) [T]

EDC 14-371 (GENT)

EDC 12-002 (GENT)

EDC 12-004 (GENT)

EDC 12-001 (GENT) [T]

NAL 348 (GENT)

AB 320 (GENT)

ADK 2927 (BR)

ADK 2968 (BR)

JR Z366 (GENT)

JR Z439 (GENT)

JR Z310 (GENT) [T]

JD 959 (BR)

BB 3466 (GENT) [T]

TS 1116 (GENT) [T]

MH 201176 (GENT)

CS 2889 (GENT)

EDC 12-176 (GENT)

EDC 12-071 (GENT)

EDC 14-284

AV 11-022 (GENT)

JN 2011-012 (GENT)

TENN 065929 (TENN)

EDC 14-501 (GENT, MFLU)

TS 0705 (GENT) [T]

AV 94-063 (GENT) [T]

COUNTRY

Malawi

Cameroon

Congo

Malawi

North

America

Burundi

Cameroon

Thailand

Togo

Cameroon

Guinea

Benin

Congo

Zambia

Tanzania

Mozambique

Zimbabwe

Cameroon

Gabon

Tanzania

Vietnam

North

America

Thailand

Tanzania

Burundi

ITS

KR364012

KR364067

KR364074

KR364024

HQ318285

KR364118

KR364119

KR364069

KR364084

HG426469

KX499389

KX499392

KX499390

KR364061

KX499391

AM113441

KR364132

KR364113

KX499393

KX499399

KX499398

KR364027

KR364081

KR364014

KR364039

KR364099

KX499394

KR364070

KX499396

KX499395

FR731875

KX499397

KR364045

KR364102

KR364127

KR364038

KR364017

LSU

KR364139

KR364196

KR364203

KR364151

HQ318208

KR364244

KR364198

KR364214

KR364186

KX622761

KX622760

KR364190

KR364252

KR364243

KR364154

KR364210

KR364250

KR364166

KR364231

KR364199

KX622762

KX622763

KR364171

KR364233

KR364165

KR364144

Lactifluus persicinus sp. nov. (Cameroon) ... 473

Lactifluus sect. Rubroviolascentini

Lf. aff. rubroviolascens EDC 12-051 (GENT) Cameroon KR364066 KR364195

Lf. carmineus AV 99-099 (GENT) [T] Zimbabwe KR364131 KR364251

Lf. denigricans EDC 11-218 (GENT) Tanzania KR364051 KR364178

Lf. kigomaensis AV 11-006 (GENT) Tanzania KR364052. ~KR364179

Lf. sp. EDC 11-159 (GENT) Tanzania KR364050 KR364177

Lactifluus sect. Polysphaerophori

Lf. pegleri PAM/Mart 12-091 (LIP) Martinique KP691416 KP691425

Lf. sp. RC/Guy 09-036 (LIP) French Guiana KJ786645 KJ786550

MR/Guy 13-145 French Guiana KJ786691 KJ786595

MCA 3937 (GENT) Guyana KR364109 KR364240

Lf. veraecrucis M 8025 (ENCB) [T] Mexico KR364112 KR364241

Outgroup (Lf. subg. Lactifluus)

North

Lf. corrugis s.l. AV 05-392 (GENT) JQ753822 = KR364143

America

Lf. crocatus KVP 08-034 (GENT) Thailand HQ318243 HQ318151

Lf. vitellinus KVP 08-024 (GENT) Thailand HQ318236 HQ318144

Lf. volemus KVP 11-002 (GENT) Belgium JQ753948 KR364175

Lf. acicularis KVP 08-002 (GENT) Thailand HQ318226 HQ318132

2010, Van de Putte 2012, Van de Putte et al. 2010). Species diversity is high in

the Guineo-Sudanian domain (Maba 2015; Maba et al. 2014, 2015a, 2015b),

with around 38 Lactifluus species currently known from the area. However,

certain regions within this area remain poorly explored with potentially many

new species yet to be discovered and described. In one such region, Cameroon,

most studies have been located in the tropical rainforest (Douanla-Meli &

Langer 2009, Verbeken et al. 2008), and little is known about milkcaps in

the savannas, from which only one Lactifluus species, Lf. albomembranaceus

S. De Wilde & Van de Putte, has been described (De Crop et al. 2016).

Southern Cameroon is dominated by closed evergreen lowland forest, while

the centre of the country is dominated by deciduous woodland and shrubland

(WRI 2003). The tropical forest does not undergo a gradual change to savanna

or woodland; instead the zone between these two habitats consists of habitat

patches with abrupt borders. This transition zone is called the ‘forest-savanna

mosaic since it contains forest islands and savannas, as well as woodlands

(White 1983). In contrast to savannas and woodlands, dense tree cover

characterizes the forest islands, with the resulting competition for light leading

to an understory with much lower abundances of grasses than in the savannas

or woodlands (Azihou et al. 2013, Hoffmann et al. 2009, Natta et al. 2003).

Forest islands that occur alongside waterways are known as gallery forests.

The plant groups Caesalpinioideae (represented by Berlinia Sol. ex Hook. f.)

A7A ... Delgat & al.

and Phyllanthaceae (represented by Uapaca Baill.) dominate the Central and

West African gallery forests. These trees are ectomycorrhizal hosts, making the

humid gallery forests an ideal habitat in which to find ectomycorrhizal fungi.

A recent survey of wild mushrooms in the West region of Cameroon revealed

nine edible Lactifluus species, all collected in gallery forests (Njouonkou et al.

2016). From the same division, Lf. albomembranaceus was described (De Crop

et al. 2016). It is from this habitat that the new species described here, Lactifluus

persicinus, was discovered.

Materials & methods

Sampling

During fieldwork in 2011, 2012, and 2014 in gallery forests within the Noun division

of western Cameroon, 15 collections of Lactifluus persicinus were made.

Morphological analysis

Macroscopic characters were observed from fresh material, with colour codes

referring to Kornerup & Wanscher (1978). Microscopic characters were observed from

dried material. Spores were mounted in Melzer’s reagent and measured in side view

without ornamentation. A total of 186 spores were measured among 14 collections.

The measurements are given as [Ava-2xSDa]-Ava-Avb-|Avb+2xSDb] in which Ava =

lowest mean value for the measured collections, Avb = greatest mean value and SDa/b =

standard deviation of the lowest and greatest mean value respectively. As an indication

for spore shape, Q was also determined, where Q is the quotient of length and width

and is given as MINQ-AvQa-AvQb-MAXQ, with AvQa and AvQb the lowest and

the highest mean quotient, respectively, for the measured specimens. MINQ/MAXQ

stands for the minimum/maximum value over the quotients of all available measured

spores. The hymenium, pileipellis and stipitipellis were mounted in Congo red. The

measurements of the basidia do not include the sterigmata. Line drawings were made

with the aid of a drawing tube at following magnifications: 6000x for spores (Zeiss Axio

Scope 2 microscope), 1500x for other hymenial elements and sections (Olympus cx31

microscope). For the SEM pictures, the spores were coated in a Quorum Q150TS sputter

coater with 5 nm thick Pt/Pd (platinum palladium) and studied using a Jeol JSM-7600F

Field Emission Scanning Microscope.

Molecular analysis

DNA from dry collections was extracted using the protocol described by Nuytinck

& Verbeken (2003) with modifications described in Van de Putte et al. (2010). DNA

was extracted from fresh material using the CTAB extraction described in Nuytinck &

Verbeken (2003). Protocols for PCR amplification follow Le et al. (2007). Two nuclear

markers previously shown as informative within this subgenus (De Crop et al. 2017) were

used: (1) the internal transcribed spacer region of ribosomal DNA (ITS), comprising the

ITS1 and ITS2 spacer regions and the ribosomal gene 5.8S, using primers ITS-1F and

Lactifluus persicinus sp. nov. (Cameroon) ... 475

ITS4 (Gardes & Bruns 1993, White et al. 1990) and (2) a part of the ribosomal large

subunit 28S region (LSU), using primers LROR and LR5 (Moncalvo et al. 2000).

PCR products were sequenced using an automated ABI 3730 XL capillary sequencer

(Life Technology) at Macrogen. Forward and reverse sequences were assembled into

contigs and edited where needed with the Sequencher v5.0 software (Gene Codes

Corporation, Ann Arbor, MI, U.S.A.).

From De Crop et al. (2017) we knew that Lactifluus persicinus represented Lactifluus

subg. Pseudogymnocarpi (Verbeken) De Crop and Lf sect. Xerampelini De Crop.

The dataset for phylogenetic analyses contained sequences from Lactifluus subg.

Pseudogymnocarpi (De Crop et al. 2017) and we added more newly generated sequences

plus two GenBank sequences within Lf. sect. Xerampelini. For other sections only one

sequence per species was included. Five species representing Lf subg. Lactifluus were

used as outgroup (TABLE 1).

Sequences were aligned online using the E-INS-I strategy version of the multiple

sequence alignment program MAFFT v7 (Katoh & Toh 2008). Trailing ends were trimmed

and the alignment was manually edited when necessary in Mega 6 (Tamura et al. 2013).

The alignment can be obtained from the first author and TreeBASE (Submission ID

19693). The alignment was partitioned into partial 18S, ITS1, 5.88, ITS2 and partial 28S.

Maximum likelihood (ML) analyses were conducted with RAXML v8.0.24 (Stamatakis

2014), where a ML analysis was combined with the Rapid Bootstrapping algorithm with

1000 replicates under the GTRCAT option (Stamatakis et al. 2008). All analyses were

performed on the CIPRES Science Gateway (Miller et al. 2010).

Results

The molecular results placed the new species in Lf. subg. Pseudogymnocarpi

and Lf sect. Xerampelini. Its sister clade contains collections previously

identified as Lf goossensiae (Beeli) Verbeken from Guinea and Lf. cf.

pseudovolemus (R. Heim) Verbeken from Benin. The new species, described

below as Lf. persicinus, is morphologically very similar to the collections within

this sister clade with which it is compared.

Lactifluus persicinus Delgat & De Crop, sp. nov. FIGS 1-3

MycoBAnk MB 817873

Differs morphologically from its sister clade (collections AB 320, ADK 2927 and ADK

2968) by its shorter basidia.

TYPE: Cameroon, Western region, Noun division, Koutaba subdivision, Mamevouo

village, gallery forest in savannah region, Uapaca guineensis, 5°41’01”N 10°49’28”E,

1126 m, 8 May 2012, EDC 12-001 (Holotype, GENT)

Erymo ocy: Referring to the peach-like color and surface of the basidiocarp.

PiLeus 37-73 mm diam., firm, plano-concave with depressed centre to deeply

infundibuliform; margin straight to inflexed, broadly grooved (c. 2-3 mm)

476 ... Delgat & al.

Fic. 1. Lactifluus persicinus basidiocarps: a. holotype, EDC 12-001; b. EDC 14-349; c. EDC 12-002;

d. EDC 14-349; e. EDC 14-380; f. EDC 14-380. (Photos by E. De Crop).

reaching halfway across the pileus; surface chamois-leather-like, slightly

velutinous when young, cream yellow to orange to pink (5A3-6A2), some pilei

slightly darker (6B4-7C4), locally more pink or more yellow, concentrically

wrinkled near margin. STIPE 32-48 x 8-14 mm, cylindrical, sometimes tapering

downwards or curving at the base; surface slightly irregular, almost smooth,

very soft (softer than pileus), slightly velutinous or chamois-leather-like, almost

concolourous with pileus but slightly paler, cream yellow-orange (5A3, 5A4

to 6A3), sometimes with a pinkish tinge, pale at top, becoming darker orange

towards the base (5A5-5B5). LAMELLAE decurrent with rather long teeth,

very broad, thick, strongly transvenose and slightly intervenose, sometimes

bifurcating towards the margin, brittle, distant (2+3-5 L+lIl/cm), paler than

pileus, cream yellow with pinkish tinge (4A3-5A2); edge concolourous and

entire. CONTEXT white, firm, unchanging, unchanging with guaiac, slightly

pink with FeSO,. SMELL not remarkable. Taste mild. Larex abundant, watery

white, unchanging, taste mild.

Basip1osPorREs broadly ellipsoid to ellipsoid, rarely slightly elongate, 6.9-

9.2-10.9-12.8 x 5.7-6.7-7.7-9.4 um (Q = 1.10-1.33-1.48-1.70); ornamentation

amyloid, composed of low (1 um at most) elongated warts, aligned or connected

Lactifluus persicinus sp. nov. (Cameroon) ... 477

Fic. 2. Lactifluus persicinus: a. basidiospores; b. basidia; c. section through the pileipellis; d. sterile

elements from the hymenium; e. pileipellis hairs. Scale bar = 10 um. (Line drawings by L. Delgat).

by fine connective lines, forming an incomplete reticulum, with only few isolated

warts; plage sometimes distinct and with a central amyloid spot. Basip1A

45-74(-90) x (8-)8.5-11.5(-12.5), cylindrical to subclavate, 4-spored, rarely

2-spored, slightly thick-walled. STERILE ELEMENTS abundant, 21-60 x 3.5-7

um, some emergent, majority not emergent, cylindrical, septate, with rounded

apex, wall slightly thickened. PLEUROCySTIDIA absent. PLEUROPSEUDOCYSTIDIA

478 ... Delgat & al.

P /

tif

Fic. 3. Lactifluus persicinus (holotype, EDC 12-001) basidiospores (SEM): a. overview; b. detail of

basidiospores. Scale bars = 1 um.

scarce, 6-10 um diam., cylindrical with rounded apex, often remarkably

emergent, sometimes not emergent. LAMELLAR EDGE fertile. HYMENOPHORAL

TRAMA cellular, with lactifers and sphaerocytes. PILEIPELLIS a lampropalisade;

elements of the suprapellis 19-65 x 3-11 um, cylindrical to cylindrical with

broadened base, often septate, thick-walled; subpellis composed of slightly

thick-walled globose/isodiametric cells (10-30 um diam.), up to 80 um thick.

STIPITIPELLIS a lamprotrichoderm to lamprotrichopalisade, with many parallel

hyphae, part of them ascending; terminal elements more abundant than in the

pileipellis, occurring in tufts, cylindrical, cylindrical with a bulbous base or

broadly ellipsoid to globose, often septate, slightly thick-walled.

Uses - Lactifluus persicinus is used as food by Bamoun people in the Noun

division of the west region of Cameroon.

Loca NAME - Lactifluus persicinus is locally called “Puo mbum mon (pw3’

mbttm mon)” by Bamoun people. “Puo” is a general word for mushroom.

“Mbum mon” is the local name of a herbaceous Euphorbia species, namely

Euphorbia heterophylla L., which is an annual plant with white milky latex in

all parts of the plant. This name is related to the fact that Lactifluus persicinus

produces abundant white latex like Euphorbia species. However, this name also

seems to be used for other milkcap species.

EcoLocy - found in Cameroon in the ‘forest-savanna mosaic’ zone in

gallery forests with Uapaca guineensis Mull. Arg.

DISTRIBUTION — known only from Cameroon.

ADDITIONAL SPECIMENS EXAMINED - CAMEROON, WESTERN REGION, Noun

division, Koutaba subdivision, Mamevouo village, gallery forest in savannah region,

Uapaca guineensis, 5°41’01”N 10°49’28”E, 1126 m, 8 May 2012, EDC 12-002, EDC 12-

003, EDC 12-004 (GENT); 5°39’12”N 10°50’54”E, 1062 m, 10 May 2012, EDC 12-050

(GENT); 5°40’59”N 10°49’29”E, 1122 m, 27 May 2014, EDC 14-349 (GENT); 5°40’48”N

Lactifluus persicinus sp. nov. (Cameroon) ... 479

10°49’28”E, 1139 m, EDC 14-361 (GENT); 5°39’27”N 10°50’57”E, 1063 m, 29 May

2014, EDC 14-371 (GENT); 5°39’26”N 10°50’57”E, 1113 m, EDC 14-374 (GENT);

5°39’10”N 10°51’00”E, 1117 m, EDC 14-376 (GENT); 5°39’10”N 10°51’01”E, 1093 m,

EDC 14-378 (GENT); 5°38’60”N 10°51’05”E, 1117 m, EDC 14-380 (GENT); 5°38’58”N

10°51’03”E, 1120 m, EDC 14-385, EDC 14-386 (GENT); 5°39’37”N 10°50’48”E, 1099

m, 22 August 2011, NAL 348 (GENT).

Discussion

Lactifluus persicinus is recognizable in the field as a typical Lactifluus

because of the chamois-leather-like surface of the cap. This is confirmed under

the microscope by the cellular hymenophoral trama and the lampropalisade

pileipellis structure. Molecular data also confirm placement within Lactifluus

(Fic. 4).

Within the genus, Lactifluus persicinus belongs to Lf subg. Pseudogymnocarpi,

which is supported by molecular data (De Crop et al. 2017), as well as by

morphological characters, such as the lampropalisade pileipellis structure,

yellow/orange cap colour, and absence of colour reactions of the latex as in

most species of this subgenus.

Lactifluus persicinus is placed within the section Lf sect. Xerampelini,asection

containing exclusively African species (Fic. 4). It also fits morphologically

within this clade, which is characterized by yellowish-orange to reddish-brown

cap colours, palisade-like pileipellis structure, absence of clear cystidia, and

generally low ornamented spores (usually not higher than 0.2 um) that are

verrucose or form a more or less complete reticulum.

The sister clade of Lf persicinus contains collections previously identified

as Lf. goossensiae and Lf. cf. pseudovolemus (Fic. 4) from Guinea and Benin.

However, Lf pseudovolemus is known only from Madagascar, and several

macro- and microscopic characters mentioned in the description (Heim 1938)

are not concordant with characters of the collections in this clade. In addition,

we have studied the type material of Lf goossensiae and this species also seems

to differ both macro- and microscopically from the sequenced collections in

this clade (e.g. the type of Lf goossensiae has smaller spores). It seems likely

that this sister clade represents neither Lf pseudovolemus nor Lf. goossensiae.

As it was not possible to obtain sequences from the types of Lf pseudovolemus

and Lf goossensiae to test the two species molecularly, the identities of the

specimens assigned to the sister clade remain unconfirmed.

Although Lactifluus persicinus is clearly delimited from its sister clade

molecularly, morphologically the two clades are difficult to distinguish from

each other and might be considered (pseudo) cryptic species. Macroscopically

480 ... Delgat & al.

Lf. persicinus Epc 14-371 Cameroon

Lf. persicinus €0c 12-002 Cameroon

98 Lf. persicinus EDC 12-004 Cameroon

Lf. persicinus EDC 12-001 Cameroon Type

100 Lf. persicinus NAL 348 Cameroon

99, Uncultured ectomycorrhiza Am113441.1 Guinea

Lf. goossensiae AB 320 Guinea

Lf. cf. pseudovolemus Avk 2968 Benin

100] iF of pseudovolemus AdK 2927 Benin

Lf. kivuensis jr 2366 Congo

100] Lf kivuensis sr 2439 Congo

100 100 Lf. kivuensis JR 2310 Congo Type

Lf. rubiginosus J0-959 Congo

100! Lf. rubiginosus BB 3466 Zambia Type

Lf. xerampelinus Ts 1116 Tanzania Type

92 100_| Lf xerampelinus mx 201176 Mozambique

Lf. xerampelinus cs 2889 Zimbabwe

Lf. sp. Epc 12-176 Cameroon

Lf. sp. E0c 12-071 Cameroon

Lf. sp. EDC 14-284 Cameroon

Uncultured ectomycorrhizal fungus FR731875.1 Gabon

Lf. sp. av 11-022 Tanzania

Lf. cf. pseudogymnocarpus Av 05-085 Malawi

Lf. cf. pumilus Eoc 12-066 Cameroon

91 | Lf gymnocarpoides av 05-184 Malawi

400 Lf. fongisporus av 94-557 Burundi Type

Lf. gymnocarpoides J0-885 Congo

Lf. medusae EDC 12-152 Cameroon

97 Lf. luteopus av 94-463 Burundi Type

99 Lf. hygrophoroides av 05-251 North America

7 Lf. pseudoluteopus FH 12-026 Thailand

100 Lf. sudanicus av 11-174 Togo Type

Lf. sect. Xerampelini

100

Lf. sect. Pseudogymnocarpi

Lf. sp. JN 2011-012 Vietnam

100 Lf. sp. TENN 065929 North America

100 Lf.sp. EDC 14-501 Thailand | Unnamed Clade

Lf. volemoides Ts 0705 Tanzania Type

Lf. aurantiifolius av 94-063 Burundi Type | Lf. sect. Aurantiifolii

97; Lf. kigomaensis av 11-006 Tanzania

99 Lf. sp. Epc 11-159 Tanzania

Lf. carmineus av 99-099 Zimbabwe Type Lf. sect. Rubroviolascentini

Lf. denigricans E0c 11-218 Tanzania

Lf. aff. rubroviolascens EDC 12-051 Cameroon

99 Lf. pegleri PAM/Mart 12-091 Martinique

97 Lf. veraecrucis Singer M 8025 Mexico Type

88 Lf. sp. MR/Guy 13-145 French Guiana Lf. sect. Ploysphaerophori

100 Lf. sp. RC/Guy 09-036 French Guiana

Lf. sp. mca 3937 Guyana

Lf. crocatus KvP 08-034 Thailand

Lf. corrugis s.|. av 05-392 North America

Lf. vitellinus Kvp 08-024 Thailand

Lf. acicularis KvP 08-002 Thailand

Lf. volemus kvp 11-002 Belgium

100

Outgroup (Lf. subg. Lactifluus)

100

0.09

Fic. 4. Overview Maximum Likelihood tree of Lactifluus subg. Pseudogymnocarpi, based on

concatenated ITS and LSU sequence data. ML bootstrap values >70% are shown.

the species look alike; colours and lamellae are similar, as well as the fruit

body shape, cap surface, and the latex. Microscopically they are also similar.

The spore sizes correspond—7.9-9.3-10.7-13.8 x 5.9-6.8-7.9-9.0 um (sister

clade) vs. 6.9-9.2-10.9-12.8 x 5.7-6.7-7.7-9.4 um (Lf persicinus)—as do

the spore shapes—Q = 1.18-1.35-1.39-1.66 (sister clade) vs. Q = 1.10-1.33-

1.48-1.70 (Lf. persicinus). Basidia sizes overlap—55-90 x 9.5-11.5 um (sister

clade) vs. 45-74(-90) x (8-)8.5-11.5(-12.5) um (Lf. persicinus). However, the

average basidial length differs substantially—74 x 10 um (sister clade) vs. 56

x 10 um (Lf. persicinus). So the sister clade has on average much longer and

more slender basidia than Lactifluus persicinus. Collections of the sister clade

also have similar sterile elements, which are often septate and slightly thick-

walled, and the terminal elements of the pilei- and stipitipellis also look alike.

In addition, they were collected in the same kind of habitat, namely in gallery

Lactifluus persicinus sp. nov. (Cameroon) ... 481

forests. However, all currently known collections from Lactifluus persicinus

were found in gallery forests dominated by Uapaca guineensis, while the three

sister clade collections were found in gallery forests dominated by Uapaca

somon [= U. togoensis Pax].

In summary, while morphologically very similar, these species are

genetically distinct and can be morphologically distinguished from each

other when the average basidium length is compared. These species could be

considered pseudocryptic species; at first they seemed indistinguishable, but

after the phylogeny revealed two distinct species, detailed morphological study

revealed (a) character(s) that can be used to morphologically distinguish them

from each other. This phenomenon is widespread throughout Lactifluus and

considered typical for this genus with its high molecular diversity and relatively

low morphological diversity. Many species complexes occur in Lf subg.

Lactifluus, Lf’ subg. Gymnocarpi, Lf. sect. Pseudogymnocarpi, etc. (De Crop

2016, Stubbe 2012, Van de Putte 2012) and as with most of these, the genetic

distance between Lactifluus persicinus and its sister clade is rather low, as can

be seen from the short—although well-supported—branches between them

(Fra. 4). This may suggest a relatively recent divergence between species as well

as insufficient time for significant morphological divergence to develop.

Acknowledgments

The first author was funded by a doctoral scholarship of the Special Research Fund

(BOF). The 2014 survey in Cameroon was financially supported by the Research

Foundation Flanders (FWO, grant V416214N) and the Leopold HI fund. We would like

to express our gratitude to all who helped during field work. The authors would also

like to thank André De Kesel, for providing the Lf cf. pseudovolemus collections with

his macroscopic notes and pictures. Thanks Bertie-Joan Van Heuven and Yoran Toonen

for making the SEM pictures. We would also like to thank Ursula Eberhardt and Andy

Taylor for reviewing this paper.

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MY COTAXON

July-September 2017— Volume 132, pp. 485-493

https://doi.org/10.5248/132.485

Distoceratosporella digitiformis gen. & sp. nov. from Brazil,

Alcornia sessilispora gen. & comb. nov.,

and three new Distoceratosporella combinations

JOSIANE SANTANA MONTEIRO’, SHEILA MIRANDA LEAO-FERREIRA’,

Luis FERNANDO PASCHOLATI GUSMAO’, Paut M. Kirk?

& RAFAEL FE. CASTANEDA-RUIZ 3

! Universidade Estadual de Feira de Santana,

Avenida Transnordestina, s/n, Novo Horizonte, 44036-900, Feira de Santana, Brazil

? Biodiversity Informatics & Spatial Analysis, Jodrell Laboratory,

Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, UK

* Instituto de Investigaciones Fundamentales en Agricultura (INIFAT) Alejandro de Humboldt,

Calle 1 Esq. 2, C.P. 17200, Santiago de Las Vegas, C. Habana, Cuba

* CORRESPONDENCE TO: /gusmao@uefs. br

ABSTRACT—Alcornia is proposed as a new monotypic genus differentiated from Parapericonia

by its tretic conidiogenous cells and rhexolytic conidial secession; a new combination

Alcornia sessilispora is also proposed, based on P. sessilispora. Distoceratosporella digitiformis

is proposed as a new genus and species distinguished from Ceratosporella by its distoseptate

conidia. These taxa are described and illustrated. Additionally, the new combinations

Distoceratosporella amazonica, D. hernandezii, and D. longiramosa are proposed, all with

Ceratosporella basionyms.

Key worps—Ascomycota, conidial fungi, hyphomycetes, taxonomy, tropics

Introduction

The diversity of conidial fungi in the Brazilian semiarid region has received

little attention. During a survey of hyphomycetes associated with plant

materials from Bahia state, two conspicuous fungi were collected. One fungus

showed remarkable differences from all previously described genera (Seifert et

al. 2011), and the second, although an identifiable species, differed significantly

486 ... Monteiro & al.

from all other species of the genus in which it had originally been described.

The two species are proposed here as the types of two new genera, Alcornia and

Distoceratosporella.

Materials & methods

Samples of leaf litter and decaying fruits were collected in Abaira and Serra do

Ramalho, Bahia state, Brazil. The samples were placed in paper bags for transport to

the laboratory, where they were placed in Petri dish moist chambers and stored at 25°C

for 30 days in a 170 L polystyrene box with 200 mL sterile water plus 2 mL glycerol

(Castafieda-Ruiz et al. 2016). Mounts were prepared in PVL (polyvinyl alcohol and

lactic acid) and measurements were made at a x1000 magnification. Micrographs

were obtained with an Olympus BX51 microscope equipped with bright field and

Nomarski interference optics. The specimens, including the holotype, are deposited in

the Herbarium of Universidade Estadual de Feira de Santana, Bahia, Brazil (HUEFS).

Taxonomy

Alcornia J.S. Monteiro, $.M. Leao, Gusmao, P.M. Kirk & R.F. Castafieda, gen. nov.

INDEX FUNGORUM IF 553580

Differs from Parapericonia by its tretic conidial ontogeny and rhexolytic conidial

secession

TYPE SPECIES: Parapericonia sessilispora Alcorn & P.M. Kirk [= Alcornia sessilispora

(Alcorn & P.M. Kirk) J.S. Monteiro et al.]

Erymo.oey: Latin, Alcornia, named in honor to John L. Alcorn, an Australian

mycologist for his contribution to the study of fungal diversity.

CoNIDIOMATA on the natural substratum sporodochial, punctiform, scattered;

brown, dark brown, or black. CONIDIOPHORES macronematous, mononematous,

erect, unbranched or slightly branched, cylindrical, with a distinct constriction

near the base, flexuous, sub-coiled, undulated, septate, verruculose, pale

brown or brown. CONIDIOGENOUS CELLS polytretic, integrated cylindrical,

determinate, intercalary, rarely terminal, brown. Conidial secession rhexolytic.

Conliplia solitary, pleurogenous, globose, unicellular, verruculose or verrucose

with a basal frill, dark brown to dark reddish-brown.

Alcornia sessilispora (Alcorn & P.M. Kirk) J.S. Monteiro, S.M. Ledo, Gusmao,

P.M. Kirk & R.F. Castaneda, comb. nov. Figs 1

INDEX FUNGORUM IF 553581

= Parapericonia sessilispora Alcorn & P.M. Kirk, Trans. Br. Mycol. Soc. 85(3): 561 (1985).

CONIDIOMATA on the natural substratum rudimentary sporodochial,

punctiform, scattered; brown, dark brown or black. Mycelium mostly

superficial, composed of septate, verruculose, somewhat leprous, brown

The new genera Alcornia e& Distoceratosporella ... 487

Fic. 1. Alcornia sessilispora (HUEFS 210449). A. Conidia with small peg (arrow). B. Detail

of conidiogenous cells with pores (arrows head) and conidia with small peg (arrow).

C. General aspect of conidiophores. D. Detail of basal cell of conidiophore. Scale bars: A = 5 um;

B, D = 10 um; C = 50 um.

488 ... Monteiro & al.

hyphae, 2-4 um diam. CONIDIOPHORES macronematous, mononematous,

arising in fascicles from the conidiomata, erect, unbranched or slightly

branched above, flexuous, sub-coiled, undulating toward the apex and

distinctly constricted near the base, 8-14-septate, verruculose, pale brown to

mid brown, <500 um long, 5-6 um wide, proximal part below the constriction

7-25 um long. CONIDIOGENOUS CELLS polytretic, integrated, verruculose,

cylindrical, determinate, intercalary, rare terminal, brown, 11-20 x 2.5-3.5

um. Conidial secession rhexolytic. Conip1A solitary, pleurogenous, globose,

unicellular, verruculose or verrucose, 5-7 um diam, dark brown to dark

reddish-brown, with a pale brown, 1-1.5 um long basal frill.

SPECIMEN EXAMINED: BRAZIL. Banta, Serra do Ramalho, on decaying fruit of

unidentified plant, 13°32’S 43°47’W, alt. 450-600 m, 16 March 2008; coll. S.M. Leao-

Ferreira (HUEFS 210449).

CoMMENTs: Parapericonia M.B. Ellis, typified by P. angusii M.B. Ellis,

is characterized by pulvinate, punctiform sporodochial conidiomata

arising from superficial and immersed stromata. The conidiophores are

macronematous, mononematous, unbranched or branched, straight or

flexuous, uncinate at the apex, constricted near the base, pale to mid brown,

verruculose toward the apex. The conidiogenous cells are monoblastic

or polyblastic, turbinate or clavate, discrete, laterally developed on the

conidiophores and branches, solitary or short moniliform and produce

solitary or blastocatenate, spherical, unicellular conidia verruculose, dark

brown that seceding schizolytically (Ellis 1976). Sporotretophora Whitton et

al. resembles Alcornia in its conidiophore arrangement, enteroblastic tretic

conidiogenesis, and unicelled conidia; but Sporotretophora has monotretic

conidiogenous cells and schizolytic secession of conidia (Whitton et al.

2012).

The conidiomata in Alcornia are considered rudimentarily sporodochial

but lacking immersed or superficial stromata and with the conidiophores

arising more or less in a radial fascicle from a tuft formed by branched and

anastomosing hyphae. Its conidiogenous cells are polytretic, integrated,

intercalary, and the conidia attach to the conidiogenous loci by a diminutive

peg, 1-1.5 um long that remains attached to the conidia after rhexolytic

conidial secession. The three important diagnostic characters separating

Alcornia from Parapericonia include: a) integrated (not moniliform)

conidiogenous cells, b) tretic conidiogenous cells, and c) rhexolytic conidial

secession (Alcorn & Kirk 1985). These differences support a new genus and

new combination for Parapericonia sessilispora.

The new genera Alcornia & Distoceratosporella ... 489

ALA

Fic. 2. Distoceratosporella digitiformis (holotype, HUEFS 216663). A. Conidia. B. Conidiophores,

conidiogenous cells, and conidia. Scale bars = 10 um.

Distoceratosporella J.S. Monteiro, Gusmao & R.E. Castafieda, gen. nov.

INDEXFUNGORUM IF553582

Differs from Ceratosporella by its distoseptate conidia.

TYPE SPECIES: Distoceratosporella digitiformis J.S. Monteiro et al.

EryMo_oey: Latin, disto-, referring to the type of septa, + -ceratosporella referring to

the genus Ceratosporella.

490 ... Monteiro & al.

Conidial fungi. CoLonizs on the natural substratum effuse; brown, dark

brown, or black. CONIDIOPHORES macronematous, mononematous, erect,

unbranched, septate, brown or black. CONIDIOGENOUS CELLS monoblastic,

cylindrical, integrated, with enteroblastic, percurrent extensions, brown.

Conidial secession schizolytic. Conrp1 solitary, acrogenous, stauroconidium

or cheiroid, with 2 or more distoseptate rows of cells, pale brown to dark brown,

smooth or verrucose.

Distoceratosporella digitiformis J.S. Monteiro, Gusmao, & R.F. Castafieda,

sp. nov. FIGS 2, 3C

INDEX FUNGORUM IF 553583

Differs from Ceratosporella bicornis and all other Ceratosporella spp. by its distoseptate,

digitiform conidia.

Type: BRAZIL. Banta: Abaira, Mata da Tijuquinha (Catolés), on decaying leaves of

unidentified plant, 13°13’S 41°37’W, 9 January 2015, coll. J.S. Monteiro (Holotype:

HUEFS 216663).

EryMo_oey: Latin, digitiformis, meaning finger-like, referring to the two rows of cells

in the conidia.

Cotonigs on the natural substratum effuse, hairy, dark brown. Mycelium

mostly superficial, composed of branched, smooth, brown hyphae, 2-3 um

diam. CONIDIOPHORES macronematous, mononematous erect, straight,

unbranched, 1-4-septate, 20-40 x 3-4 um, brown to dark brown at the base,

pale brown toward the apex, smooth. CONIDIOGENOUS CELLS monoblastic,

cylindrical or doliiform, integrated, indeterminate with 1-3 enteroblastic

percurrent extensions, pale brown, smooth, 4-15 x 3.5-4 um. Conidial secession

schizolytic. ConIpIA acrogenous, solitary, 32-43 x 12-16 um, digitiform, with

a doliiform or very short cylindrical basal cell, 4-6 x 3-4 um, brown, smooth,

composed of two parallel, cylindrical, brown, 4-8-distoseptate, smooth row of

cells, 18-32 x 6-8 um.

ComMENTs: Ceratosporella Hohn., typified by Triposporium bicorne Morgan [=

C. bicornis (Morgan) Héhn.] (Fie. 3a), is distinguished by macronematous,

mononematous, unbranched conidiophores and monoblastic, cylindrical

or doliiform, terminal, integrated conidiogenous cells with one or several

enteroblastic percurrent extensions. The conidia are solitary, acrogenous,

stauroconidia or cheiroid, euseptate, brown. The remaining 13 Ceratosporella

species have euseptate conidia, agreeing with the generic concept provided

by Hoéhnel (1923) and treated by Castaneda-Ruiz et al. (1996), Kuthubutheen

& Nawawi (1991), Monteiro & Gusmao (2014). Distoceratosporella closely

The new genera Alcornia e& Distoceratosporella ... 491

Fic. 3. Ceratosporella and Distoceratosporella species: conidia of type specimens. A. Ceratosporella

bicornis (Hughes 1951); B. Distoceratosporella amazonica (Monteiro & Gusmao 2014);

C. Distoceratosporella digitiformis (this paper); D. Distoceratosporella hernandezii (Monteiro &

Gusmao 2014); E. Distoceratosporella longiramosa (Castaneda-Ruiz 1988). Scale bars: A, B, D, E

= 20 um; C = 10 um.

492 ... Monteiro & al.

resembles Ceratosporella but can be separated by its distoseptate conidia. Similar

criteria have been used to separate other generic pairs, such as Corynespora

Giissow and Solicorynespora R.F. Castafteda & W.B. Kendr., Sporidesmiella

P.M. Kirk and Sporidesmium Link, Ellisembia Subram. and Sporidesmium,

and Minimelanolocus R.F. Castaheda & Heredia and Pseudospiropes M.B. Ellis

(Castaneda-Ruiz & Kendrick 1990, Kirk 1982, Subramanian 1992, Castafheda-

Ruiz et al. 2001).

New combinations for three Ceratosporella species are proposed in

Distoceratosporella, based on their distoseptate conidia:

Distoceratosporella amazonica (J.S. Monteiro & Gusmao) J.S. Monteiro,

Gusmao & R.E Castaneda, comb. nov. Fic. 3B

INDEX FUNGORUM IF 553584

= Ceratosporella amazonica J.S. Monteiro & Gusmao, Nova Hedwigia 98: 483 (2014).

Distoceratosporella hernandezii (J.S. Monteiro & Gusmao) J.S. Monteiro,

Gusmao & R.E Castaneda, comb. nov. Fic. 3p

INDEX FUNGORUM IF 553585

= Ceratosporella hernandezii J.S. Monteiro & Gusmao, Nova Hedwigia 98: 483 (2014).

Distoceratosporella longiramosa (R.F. Castafieda) R.F. Castafieda,

J.S. Monteiro & Gusmao, comb. nov. FIG. 3E

INDEX FUNGORUM IF 553586

= Ceratosporella longiramosa R.F. Castaneda, Fungi Cubenses III: 2 (1988).

= Actinocladium longiramosum (R.F. Castafieda) R.F

Castaneda, Mycotaxon 60: 278 (1996).

Acknowledgments

We are indebted to Dr. Jian Ma and Dr. De-Wei Li for critical review. The authors

thank the National Council for Scientific and Technological Development (CNPq)

(Proc. 451991/2016-8; 303062/2014-2; 154329/2016-9). RFCR is grateful to OSDE,

Grupo Agricola from Cuban Ministry of Agriculture and Programa de Salud Animal y

Vegetal (project P131LH003033). Dr. Lorelei L. Norvell’s editorial review and Dr. Shaun

Pennycook’s nomenclature review are greatly appreciated.

Literature cited

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561-564. https://doi.org/10.1016/S0007-1536(85)80063-2

Castafieda-Ruiz RF, Kendrick B. 1990. Conidial fungi from Cuba: I. University of Waterloo

Biology Series. 33: 1-62.

Castafeda-Ruiz RF, Guarro J, Cano J. 1996. Notes on conidial fungi. X. A new species of

Ceratosporella and some new combinations. Mycotaxon 60: 275-281

Castafieda-Ruiz RF, Heredia G, Reyes M, Arias RM, Decock C. 2001. A revision of the genus

Pseudospiropes and some new taxa. Cryptogamie, Mycologie 22: 1-18.

The new genera Alcornia e& Distoceratosporella ... 493

Castafieda-Ruiz RF, Heredia G, Gusmao LFP, Li DW. 2016. Fungal diversity of Central and South

America. 197-217, in: DW Li (ed.). Biology of Microfungi. Springer International Publishing.

https://doi.org/10.1007/978-3-319-29137-6_9

Ellis MB. 1976. More dematiaceous hyphomycetes. Kew, Commonwealth Mycological Institute.

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zusammengestellt und herausgegeben von Jos. Weese. Centralblatt fir Bakteriologie,

Parasitenkunde und Infektionskrankheiten, 2. Abth., 60: 1-26.

Kirk PM. 1982. New or interesting microfungi VI. Sporidesmiella gen. nov. (hyphomycetes).

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https://doi.org/10.1016/S0007-1536(82)80040-5

Kuthubutheen AJ, Nawawi A. 1991. A new species of Ceratosporella and Triposporium

lambdaseptatum (Matsush.) comb. nov. from Malaysia. Mycological Research 95: 158-162.

https://doi.org/10.1016/S0953-7562(09)81005-8

Monteiro JS, Gusmao LFP. 2014. Two new species of Ceratosporella (anamorphic fungi) from

Brazilian Amazon forest. Nova Hedwigia 98: 481-490.

https://doi.org/10.1127/0029-5035/2014/0165

Seifert K, Morgan-Jones G, Gams W, Kendrick B. 2011. The genera of hyphomycetes. CBS

Biodiversity Series 9. 997 p.

Subramanian CV. 1992. A reassessment of Sporidesmium (hyphomycetes) and some related taxa.

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Whitton SR, McKenzie EHC, Hyde KD. 2012. Anamorphic fungi associated with Pandanaceae.

125-353, in: SR Whitton et al. (eds). Fungi associated with Pandanaceae. Fungal Diversity

Research Series 21. https://doi.org/10.1007/978-94-007-4447-9_4

MY COTAXON

July-September 2017— Volume 132, pp. 495-502

https://doi.org/10.5248/132.495

First report of Co/letotrichum fioriniae infection of hazelnut

ARZU SEZER’, FATMA SARA DOLAR? & FILIz UNAL?

'Ministry of Food Agriculture and Livestock, Hazelnut Research Institute,

PK: 46, 28200 Giresun, Turkey

?Department of Plant Protection, Faculty of Agriculture, University of Ankara,

06110 Ankara, Turkey

’Ministry of Food Agriculture and Livestock, Central Plant Protection Research Institute,

Ankara, Turkey

* CORRESPONDENCE TO: arsezer@gmail.com

ABSTRACT—In a 2008-09 study of hazelnut fruit cluster diseases in Ordu, Giresun, and

Trabzon provinces of Turkey, brown to black sunken, circular to irregular lesions and

rots were observed, usually at the bottom end of the hazelnut cluster, close to the pedicel.

Colletotrichum species were most frequently isolated from the lesions, and 13 isolates were

identified morphologically as C. fioriniae, and the identity of one of them was confirmed

by molecular analyses. Pathogenicity tests were conducted under laboratory, greenhouse,

and field conditions, and symptoms matching the original field symptoms were observed

on leaves, fruit clusters, and pedicels. This is the first report of C. fioriniae as a pathogen of

hazelnut (Corylus avellana) and the first record of C. fioriniae from Turkey.

Key worps—Black Sea region, Colletotrichum acutatum, Glomerellaceae

Introduction

Hazelnut (Corylus avellana L.) is one of the most important tree nut crops

in Turkey, which with 80% of world production is the leading exporter of

hazelnuts (FAOSTAT 2011). Hazelnut production in Turkey in the Black Sea

region extends from the Georgia border in the east to Istanbul in the west.

Surveys were carried out in 2008-09, on 221 hazelnut orchards located at Ordu,

Giresun, and Trabzon provinces in the Black Sea region of Turkey (Fic. 1).

Almost 50% of the surveyed orchards had symptomatic clusters with brown to

black sunken, circular to irregular lesions that can progress from husk into fruit

496 ... Sezer, Dolar & Unal

Trabzon

k Sag

cas Ord Giresun

Mediterrenaen Sea

Fic. 1: Provinces surveyed for fruit cluster disease of hazelnut in Turkey.

(Fics 2-4). Colletotrichum was the predominant genus isolated (Sezer & Dolar

2012), and 13 morphologically similar isolates were provisionally determined

as C. fioriniae.

Colletotrichum fioriniae has not previously been reported as a pathogen of

hazelnut or from Turkey.

Materials & methods

Fungal isolates

During 2008-09, fungi were isolated from symptomatic clusters from different

cultivars and locations (Sezer & Dolar 2012). Infected fruit clusters were surface-

sterilized (0.5-1% w/v sodium hypochlorite) for 1-2 min before being placed onto

potato dextrose agar (PDA, Merck) containing streptomycin. Single spore isolates

were obtained from fungal cultures incubated at 23 + 1°C with a 12 h photoperiod for

3-5 days and stored on PDA slants in tubes at 4°C. Cultures were conserved in the

Department of Plant Protection, Ankara University, Ankara, Turkey (AUPP) and the

Hazelnut Research Institute, Giresun, Turkey (HRI).

Identification of fungus

Culture morphology, growth rate, and conidial morphology were observed from

7-10-day old cultures grown on PDA (Sutton 1980, Marcelino et al. 2008, Cai et al.

2009, Hyde et al. 2009a,b, Shivas & Tan 2009). Mycelial appressorial characters were

observed from slide cultures on Potato Carrot Agar (PCA) (Sutton 1980). Shape, length,

and width of 100 conidia were measured and mean length and width were calculated.

Initial morphological fungal identification was confirmed by CABI-UK, who sequenced

the internal transcribed spacer (ITS) of the rRNA gene of isolate P39.

Additional analyses were made of partial sequences of the beta-tubulin-2 (6-tub),

glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and chitin synthase-1 (CHS-1)

Colletotrichum fioriniae on hazelnut (Turkey) ... 497

Fics 2-4: Colletotrichum fioriniae: field symptoms. 2. Small lesions on husk. 3. Large lesions on

bottom end of the fruit cluster. 4. Brownish black lesion on fruit shell under the symptomatic husk.

genes (Damm et al. 2009, 2012a,b, 2014; Weir et al. 2012), using universal primers T1/

Bt2b for B-tub, GDF1/GDR1 for GAPDH, and CHS1-79F/CHS1-354R for CHS-1 (Glass

& Donaldson 1995, Carbone & Kohn 1999, Guerber et al. 2003). For DNA extraction

approximately 300 mg mycelium were harvested and ground with liquid nitrogen in

a sterile mortar from culture medium. Genomic DNA was extracted using a Qiagen

DNeasy ° Plant Mini Kit, as specified by the manufacturer, and stored at -20°C prior

to use. PCR reaction mixtures and condition were modified from previous studies

(Aroca & Raposo 2007, Cobos & Martin 2008). The reaction mixtures of PCR, a

final volume of 50 ul, contained 5ul of 10X buffer [75 mM Tris HCl, pH 9.0, 50 mM

KCl, 20 mM (NH,)2SO,], 2 ul of 5 uM each primers, 5 ul of 1.5mM MgCl, 2 ul of

10 mM deoxynucleoside triphosphates (dNTPs), 1 U Taq polymerase (Fermatas), 5 ul

of DNA template for each reaction and 5 ul of bovine serum albumin (BSA: 10 mg/

ml). DNA amplifications were carried out in a Techne TC-5000 thermal cycler by the

following program: 94°C for 2 min, followed by 34 cycles of [denaturation (94°C for 30

s), annealing (60°C for 30 s), and (3) extension (72°C for 30 s)], and a final extension

step 10 min at 72°C. Sequencing was conducted by Genoks (Ankara, Turkey). The

nucleotide sequences were subjected to BLAST analysis (http://www.ncbi.nlm.nih.gov)

and compared to other sequences in GenBank using CLC Main Workbench 7.0.1 (CLC

Bio, Denmark). Our new sequences were deposited in GenBank.

Pathogenicity tests

Pathogenicity tests were conducted on Tombul and Kalinkara hazelnut cultivars

using detached leaves in the laboratory, detached twigs and clusters in the greenhouse,

and attached twigs in the orchard according to Sezer & Dolar (2012).

Disease assessment

For disease assessment, percentage surface area covered by lesions on leaf or fruit

cluster of hazelnut was determined. Disease severity was calculated by estimating lesion

sizes on a scale of 0 to 3, where 0 = no lesions, 1 = 1-30% of tissues affected, 2 = 31-60%

of tissues affected, and 3 = >60% of tissues affected. These scale values were converted to

498 ... Sezer, Dolar & Unal

Fics 5-12: Colletotrichum fioriniae. 5. 5-day old colony on PDA. 6. Enlarged view of spore

masses on a 5-day old colony. 7. 10-day old colony on PDA. 8. Reverse side of 10-day old

colony. 9. Unicellular conidiophores. 10. Septate conidiophores. 11. Conidia. 12. Appressoria.

Scale bars = 10 um.

disease severity values (Xi et al. 1990) based on the formula: Disease Severity % = 100

x X(no. of plants in category x category number) / (total no. of plants x max. category

number).

Results & discussion

With a 30.41% frequency among 217 fungal isolates, Colletotrichum was

the main genus isolated from symptomatic fruit clusters. Of the thirteen

Colletotrichum isolates evaluated showing similar cultural and morphological

characteristics, one (P39) was also confirmed as C. fioriniae by phylogenetic

analyses.

Colletotrichum fioriniae (Marcelino & Gouli) Pennycook,

Mycotaxon 132(1): 150. 2017. FIG. 5-12

= Glomerella acutata var. fioriniae Marcelino & Gouli, Mycologia 100: 362. 2008.

Single spore isolates developed colonies on PDA 48.6 mm diam. after 7

days and 71.3 mm diam. after 10 days; the 7- 10-day old colonies were circular,

slightly raised, dense, and cottony with aerial mycelium in compact tufts

Colletotrichum fioriniae on hazelnut (Turkey) ... 499

Fics 13-16. Colletotrichum fioriniae: hazelnut pathogenicity test symptoms. 13. On detached leaf.

14. On pedicel of the fruit cluster on detached twig. 15. Appearance of fruit cluster 20 days after

inoculation (symptomless) in orchard. 16. Symptom on fruit cluster at harvest time in orchard.

and no distinct acervuli. Rarely observed conidial masses were salmon pink.

Surface colony color was greenish gray-pink, reverse was pale brown to pink.

While the first single spore isolate cultures on PDA were distinctly pink, the

color became less intense with subsequent subculturing. Sclerotia and setae

were absent. Mycelia were branched, septate, hyaline. Conidiophores formed

directly on hyphae, 22.9 + 1.7 x 2.6 + 0.1 um (mean), hyaline, cylindrical,

usually unicellular and occasionally branched. Conidia in masses were 11.4 +

0.2 x 4.1 + 0.1 um, hyaline, guttulate, aseptate, straight, usually ellipsoid to

fusiform and acute at both ends. Mycelial appressoria (rare in slide cultures)

measuring 8.3 + 0.2 x 5.4 + 0.1 um, pale to dark brown, clavate or slightly

irregular.

SPECIMENS EXAMINED: TURKEY, ORDU PROVINCE, Persembe district, Mersin village,

from rotting Corylus avellana fruit cluster, July 2008, Arzu Sezer P39 (AUPP, HRI;

culture, IMI 500303; GenBank KU736863, KU736864, KU736865).

These morphological characteristics matched the description of Colletotrichum

froriniae (Marcelino et al. 2008, as C. acutatum var. fioriniae; Shivas & Tan 2009),

and the rRNA gene ITS sequence from C. fioriniae isolate P39 was confirmed

500 ... Sezer, Dolar & Unal

as C. fioriniae by CABI-UK (IMI 500303). Damm et al. (2012a) recommended

that both B-tub and GAPDH genes were suitable for identification of species

belonging to the C. acutatum complex. BLAST of the DNA sequences obtained

from three different gene regions of the isolate P39 showed a high identity

(>99%) with previously deposited authentic sequences of C. floriniae in

GenBank: B-tub—JN121279, AB690809, GU183268, GU183272, KJ955322,

KJ650030; GAPDH—KM252213, KM252196, KF944354, KJ954876;

CHS-1—JQ948954, KT153547.

Pathogenicity tests showed that C. floriniae produced large circular lesions

on leaf surface. On detached twigs and clusters it caused elongate lesions on the

pedicel producing salmon-orange spore masses. At the orchard, no symptoms

were observed in inoculated fruit clusters near harvest time. At harvest three

months after inoculation, large brown necrotic areas with salmon-orange

spore masses on bottom ends of fruit clusters were observed (Fics 13-16). The

fungus was re-isolated from all diseased tissues. No symptoms were observed

on the controls. Disease severity on detached leaves was 33.33% on Tombul

cultivar and 50% on Kalinkara cultivar, 77.78% on detached twigs and clusters,

and 41.67% on fruit clusters in the orchard inoculations.

Marcelino et al. (2008) originally described Colletotrichum fioriniae as a

variety of C. acutatum (with a sexual morph, Glomerella acutata var. fioriniae),

and Shivas & Tan (2009) proposed that the Colletotrichum and Glomerella

names should both be elevated to species rank. Unfortunately, only one of these

four names was validly published; subsequently, C. fioriniae has been validly

published, based on the valid basionym G. acutata var. fioriniae (Pennycook

2017). According to Shivas & Tan (2009) C. fioriniae is morphologically similar

to Colletotrichum acutatum J.H. Simmonds, which differs by having colonies

on PDA with a grey cottony aerial mycelium (pale brownish pink with dark

flecking in reverse); sparse conidiomata with masses of orange conidia; and

narrowly elliptical conidia, 9-15 x 3-4.5 um.

Previously, Colletotrichum acutatum was identified as a pathogen on

hazelnut fruit clusters with same symptoms described here in Turkey (Sezer

& Dolar 2012); C. gloeosporioides (Penz.) Penz. & Sacc. was identified as a

pathogen on leaves and stems of hazelnut in Iran (Mirhosseini-Moghaddam

& Taherzadeh 2007); and Colletotrichum sp. (among other fungi) was isolated

from hazelnut flowers/fruits affected by a disease named gray necrosis in

Italy (Belisario & Santori 2009). To our knowledge, this is the first report of

C. fioriniae as a pathogen of Corylus avellana. ‘This is also the first report of

C. fioriniae infection of a crop plant in Turkey.

Colletotrichum fioriniae on hazelnut (Turkey) ... 501

Acknowledgements

This study was supported by the Scientific Research Projects Office of the Ankara

University (Project No: 08B4347008) and Republic of Turkey Ministry of Food

Agriculture and Livestock. We are grateful to Toyozo Sato (Japan) and Bevan Weir

(New Zealand) for reviewing the manuscript and suggesting improvements. We are also

grateful to Shaun Pennycook (New Zealand) for his help in improving the manuscript.

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studying Colletotrichum. Fungal Diversity 39: 183-204.

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ascomycetes. Mycologia 91: 553-556. https://doi.org/10.2307/3761358

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from grapevines in Castilla y Leén (Spain). Phytopathologia Mediterranea 47: 20-27.

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Damm U, Cannon PF, Woudenberg JHC, Johnston PR, Weir B, Tan YP, Shivas RG, Crous PW.

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https://doi.org/10.1080/07060669009501020

MYCOTAXON

July-September 2017— Volume 132, pp. 503-512

https://doi.org/10.5248/132.503

New Calbovista, Mycena, Rhizopogon, Stictis,

and Symphyosirinia records from Turkey

IBRAHIM TURKEKUL

Faculty of Arts and Science Department of Biology, Gaziosmanpasa University,

Tokat, Turkey

* CORRESPONDENCE TO: ibrahim.turkekul@gop.edu.tr

AsBstTRACT—Calbovista subsculpta, Mycena pearsoniana, Rhizopogon rocabrunae, Stictis

radiata, and Symphyosirinia angelicae were identified amongst macrofungal samples collected

from natural habitats in Tokat and Samsun provinces between 2012 and 2015. These species

are recorded for the first time from Turkey.

KEY worDs—ascomycetes, basidiomycetes, macrofungi, taxonomy

Introduction

Checklists of larger fungi recorded in Turkey have been published by Sesli

& Denchev (2014) and Solak et al. (2015) with new records added to the

Turkish mycota reported in other papers (Akata et al. 2014; Akata & Dogan

2015, Dogan & Oztiirk 2015, Giingor et al. 2015, Kaya & Uzun 2015, Kaya

2015, Sesli et al. 2015, Sesli & Moreau 2015, Turkoglu et al. 2015, Uzun et al.

2015).

Field studies were conducted during 2012-2015 in Tokat province

(Canpolat Plateau; Baydarli Village) and Samsun province (Ladik district)

in the inland Middle Black Sea Region of Turkey. The Canpolat Plateau (alt.

c. 1300 m) is a rugged semi-arid highland area with mixed forests dominated

by Quercus pubescens and Carpinus orientalis. The Baydarli village area (alt.

c. 1500 m) also has mixed forests dominated by Quercus pubescens but a drier

climate. The Ladik district (alt. c. 1400 m) is a highland area with mixed

forests dominated by Pinus nigra.

504 ... Tiirkekul

Sesli & Denchev (2008) have attributed the high mushroom diversity

to this region to its ecological characteristics, climate, and high ambient

humidity. The aim of this study was to explore the diversity of mushrooms in

the area and add to the knowledge of the Turkish mycota.

We describe and illustrate below our collections representing

species reported for the first time from Turkey: Calbovista subsculpta

(Agaricaceae), Mycena pearsoniana (Mycenaceae), Rhizopogon rocabrunae

(Rhizopogonaceae), Stictis radiata (Stictidaceae), and Symphyosirinia

angelicae (Helotiaceae).

Materials & methods

Macrofungi were collected from Canpolat and Baydarli (Tokat), and Ladik

(Samsun) areas between 2012 and 2015. The habitat and morphological

characteristics of the macrofungal specimens were recorded and photographed with

a Canon EOS 600 D digital camera equipped with a macro lens. The collections were

examined microscopically at Gaziosmanpasa University according to Clémencon

(2009). The specimens were sectioned with a new razor blade under a Nikon

Optiphot-2 microscope; sections were mounted in diluted ammonia, stained with

Melzer’s reagent, Congo red, or Cotton blue, and examined under a Nikon trinocular

microscope. Species were identified after consultation with the literature (Phillips

1981; Moser 1983; Bon 1987; Breitenbach & Kranzlin 1984, 1991, 2000; Ellis &

Ellis 1990; Jordan 2004; Knudsen & Vesterholt 2008). All samples were stored in

the Fungarium of Department of Biology, Gaziosmanpasa University, Tokat, Turkey

(FGOU).

Taxonomy

Calbovista subsculpta Morse ex M.T. Seidl, Mycotaxon 54: 390 (1995) Fig. 1

FruIT Bopy 70-140 mm broad, <80-90 mm high; plicate and contracted

toward a rooting base, globose to pear shaped. ExopERIDIUM whitish yellow

to cream when young, the color then turns to yellow with the appearance of

white patches in yellow through maturation, 5.5-7.5 mm high, breaking up

into pyramidal warts or plates separated by parallel transverse lines; small

truncated pyramids leathery, broken into irregular 1.5-3.5 mm patches, short

brownish hairs found in the pyramid centers. ENDOPERIDIUM white to cream

when young, then yellow to orange, and finally dark brown in age. GLEBA

white to cream, becoming ochre to olivaceus, finally brown. BAsIDIOSPORES

3.5-5 um diam. (occasionally ovate), hyaline, most with a central oil-droplet.

SPECIMEN EXAMINED: TURKEY, Toxart, Baydarli village, 40°57’09”"N 37°29'50”E, 1632

m, single to gregarious in subalpine open areas near forest edge, 28 April 2013, leg. I.

Turkekul (FGOU 3927).

New macrofungal records for Turkey ... 505

Fic. 1. Calbovista subsculpta: a. basidiomata; b. basidiospores;

c. basidiospores and capillitial threads; d. pileipellis.

Scale bars: a = 10 mm; b = 10 um; c, d = 20 um.

506... Tiirkekul

CoMMENTS—'The genus Calbovista, which comprises only three species,

has not previously been recorded from Turkey, thus making our report of

C. subsculpta a new record for the country at both the specific and generic

levels. It is diagnosed by the presence of nested pyramids on the cap, with the

outer pyramid taller and the inner ones shorter, irregular shaped, truncate,

and leathery. Generally found above 900 m elevation, occasionally in alpine

areas of disintegrated rock and soil mix (Ramsey 2003). Our samples were

also collected in alpine areas at 1632 m.

= = +

5 ~ - e ~

~~ ,

Fic. 2. Mycena pearsoniana: a. basidiomata; b. basidiospores. c. basidia; d. cheliocystidia.

Scale bars: a = 10 mm; b-d = 10 um.

New macrofungal records for Turkey ... 507

Mycena pearsoniana Dennis ex Singer, Sydowia 12: 233 (1959) Fig. 2

PiLeus 10-30 mm across, convex, flattening with age, becoming plano-

convex, violet pinkish tints, paler at margin. STIPE 30-55(-75) x 1.5-3 mm,

cylindrical or slightly enlarged toward base, smooth, moist, villose at base,

cinnamon at apex, fragile to firm, Taste mild or rancid, smell raphanoid.

LAMELLAE close, narrow, adnate, whitish flushed violet. PLEUROCYSTIDIA

absent. CHEILOCYSTIDIA 35-65 x 7-13 um, cylindric with rounded apex,

smooth. Spore print white. BAsip1a 25-30 x 5-7 um, clavate to cylindric.

BASIDIOSPORES 6-8 x 3.5-5 um, inamyloid, pip-shaped.

SPECIMEN EXAMINED: TURKEY, Tokar, Canpolat village, 40°33’55”N 36°36’48”E, 1245

m, on a rotten stump of Picea orientalis, 12 November 2012, leg. I. Tirkekul (FGOU

3862).

CoMMENTS—Mycena is represented by 2186 species worldwide (www.

indexfungorum.org; 15 December 2016) and 60 species in Turkey (Sesli &

Denchev 2008). Mycena pearsoniana can be diagnosed by its 35-65 x 7-13

um cylindrical cheilocystidia and pale blue to purplish pink pileus.

Fic. 3. Rhizopogon rocabrunae: a. basidiomata; b. basidiospores.

Scale bars: a = 20 mm; b = 10 um.

Rhizopogon rocabrunae M.P. Martin,

Edic. Espec. Soc. Catalana Micol. 5: 95 (1996) Fig. 3 (a,b & c,d)

FRUIT BODY 2-5 cm across, irregularly potato-shaped, ovate to globose,

brown with reddish orange squamulose peridium, roughly sub-spherical,

covered with brownish mycelial threads. The fruit body can be fully

508 ... Tiirkekul

Fic. 3. Rhizopogon rocabrunae: c. elements of pileipellis; d. basidia and glebal element.

Scale bars: c = 20 um; d = 10 um.

embedded in the soil or somewhat extending upwards. GLEBA olivaceous

at maturity, quite solid and durable. Taste and smell not distinctive. BAstp1a

13-15 x 5-9 um, clavate to cylindric, 4—spored, with basal clamp connection.

BASIDIOSPORES 6.5-9(-10) x 2.5-3.5 um, truncate, smooth, elongate, and

some have two oil droplets.

SPECIMEN EXAMINED: TURKEY, Tokar, Canpolat village, 40°33’55”N 36°36’48’E, 1245

m, solitary or gregarious a layer of Abies alba needles, 6 November 2013, leg. I. Tiirkekul

(FGOU 4115).

CoMMENTS—Rhizopogon rocabrunae is an ectomycorrhizal species

primarily associated with Abies alba. Its most remarkable features are a

reddish orange squamulose peridium and elongated basidiospores clearly

truncated at the base.

Stictis radiata (L.) Pers., Observ. Mycol. 2: 73 (1800) Fig. 4

APOTHECIA 4-8 mm, irregularly rounded, hymenium entirely embedded

in the wood, yolk-yellow to bright orange-red, margin whitish, erect, short,

and irregular, bulging upward and splitting into lobes. HymENium variably

pale orange to yellow or white. The base of the hymenium turns blue in iodine.

Asci 150-220(-250) x 8-10 um, cylindric. AscosporEs 140-210 x 2-2.5 um,

smooth, filiform, hyaline. PARAPHYSES 150-190 x 1.5-2 um, fibrous, numerous

and very slender, and sometimes branched at the top.

New macrofungal records for Turkey ... 509

Fic. 4. Stictis radiata: a. apothecia; b. asci and paraphyses.

Scale bars: a = 10 mm; b = 20 um.

510... Tiirkekul

SPECIMEN EXAMINED: TURKEY, Samsun, Ladik village, 41°05’17”N 36°05’17’E, 734

m, on the decaying branches of deciduous trees, 9 September 2015, leg. I. Tiirkekul

(FGOU 4974).

COMMENTS—Stictis radiata is a new genus for Turkey (Sesli & Denchev

2008). Breitenbach & Kranzlin (1984) stated that it was found on many

substrates, wood, herbaceous stems, fern rachises, and including decaying

leaves. We collected our samples from decaying branches of deciduous trees.

Macroscopically, it is very similar to S. stellata, which can be distinguished by a

hymenium that does not turn blue in iodine.

Symphyosirinia angelicae E.A. Ellis,

Trans. Norfolk Norw. Nat. Soc. 18(3): 11 (1956) Fig. 5

APOTHECIA 6-10 mm diam., stalked, pale, light cream turning gray when

dried. HyMENIUM pale ocher white, light cream, smooth, slightly concave. STIPE

18-25 x 0.2-0.4 mm, tubular, tapered at the top. Ascr 80-110(-120) x 8-10

(-12) um, cylindric, inoperculate, with an arched apex becoming sub-truncate

when mature. AscosporEs 14-17 x 5-6 tum, asymmetrically fusi-lageniform,

slipper formed with one end pointed and the other rounded. PARAPHYSES

45-120 x 3-4 um, cylindrical, smooth.

SPECIMEN EXAMINED: TURKEY, Samsun, Ladik village, 40°57’07”N 35°53’36”E, 1020

m, on an infected Heracleum seed buried under litter or soil, 24 September 2015, leg. I.

Turkekul (FGOU 4992).

ComMMENTS—Symphyosirinia angelicae is a mycorrhizal species found primarily

on Heracleum seeds (Garcia & Moreau 2002). The stipe length varies according

to the depth of the host seed under the soil.

Acknowledgments

We would like to thank the Gaziosmanpasa University Research Fund (GOU-BAP:

2010/115 and 2012/048) for financial support. We would like to thank Ertugrul Sesli

(Karadeniz Technical University, Turkey) and Ilgaz Akata (Ankara University, Turkey)

for reviewing this article.

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MY COTAXON

July-September 2017— Volume 132, pp. 513-517

https://doi.org/10.5248/132.513

Tripospermum sinense sp. nov. from China

Min Q1A0S, YING HUANGS, CHU DENG, ZE-FEN YU*

Laboratory for Conservation and Utilization of Bio-resources,

Key Laboratory for Microbial Resources of the Ministry of Education,

Yunnan University, Kunming, Yunnan, 650091, P. R. China

*CORRESPONDENCE TO: zfyugm@hotmail.com

ABSTRACT— Tripospermum sinense, a new species isolated from submerged dicotyledonous

leaves, is described and illustrated. It is characterized by solitary hyaline branched conidia

composed of a short main axis and four asymmetrical lateral branches and by conidiogenous

cells cicatrized after the conidia have detached.

KEY WoRDS — aquatic hyphomycetes, morphology, taxonomy

Introduction

Tripospermum Speg. was established with T. acerinum (P. Syd.) Speg. as

its type (Spegazinni 1918). The genus is characterized by the production of

acrogenous or pleurogenous, hyaline to melanocratic, multiseptate, branched

conidia often comprising a short main axis with 2-4 straight lateral branches

(Ellis 1971). Twenty-seven species have been included in Tripospermum (Rocha

et al. 2010; Index Fungorum 2016). Tripospermum chiayiense Matsush. and

T. variabile Matsush. were originally described from Taiwan (Matsushima 1980,

1983), but there have been no reports of Tripospermum from mainland China.

Several new fungal taxa occurring on decaying leaves or branches in China

have been described recently (Bai et al. 2013; Guo et al. 2015; Li et al. 2014;

Ma et al. 2011a,b; Ren et al. 2012; Yang et al. 2011, 2012; Zhang et al. 2009).

During a study of the aquatic hyphomycetes on submerged dicotyledonous

leaves collected in Leigong Mountain, Guizhou Province, China (a Nature

‘These authors contributed these work equally.

514 ... Qiao, Huang & al.

Reserve and National Forest Park), we encountered a fungus sharing features

of Tripospermum; we describe this here as a new species, T. sinense.

Materials & methods

Submerged dicotyledonous leaves were collected from a stream in Leigong

Mountain, Guizhou Province, China. Samples were preserved in zip-locked plastic

bags for transport to the laboratory where the rotten leaves were cut into 2-4 x 2-4

cm fragments, spread onto the surface of CMA medium (20 g cornmeal, 18 g agar, 40

mg streptomycin, 30 mg ampicillin, 1000 ml distilled water), and incubated for about

10 days in Petri dishes. Single conidia were isolated for cultivation on CMA with the

aid of a BX51 microscope and sterilized needles and the cultures were morphologically

examined after incubation on CMA at 28°C for one week. A frozen culture is deposited

in the Herbarium of the Laboratory for Conservation and Utilization of Bio resources,

Yunnan University, Kunming, Yunnan, P.R. China (YMEF, formerly Key Laboratory of

Industrial Microbiology and Fermentation Technology of Yunnan).

Taxonomy

Tripospermum sinense Ying Huang & Z.F. Yu, sp. nov. PLATE 1

MycoBAnk MB 815346

Differs from Tripospermum camelopardus by its five-radiate conidia, from T. myrti by its

larger conidia, and from T: porosporiferum by its smaller conidia.

Type: PR China, Guizhou Province, Leigong Mountain Nature Reserve and National

Forest Park, 25°53”N 108°24’E, elev. 2178 m, on submerged leaves of an unidentified

dicotyledonous plant in a stream, Oct. 2013, Z.F. Yu (Holotype, YMF 1.04181 [frozen

culture]).

ETyMOLoGy: sinense refers to the country in which this species was found.

COLONIES growing on CMA, reaching about 20 mm diameter after 10 days

at 25°C. Colony effuse, brownish to dark brown, mycelium scanty superficial,

mostly in substratum immersed, composed of branched, septate, hyaline to

brown-tinged hyphae. Vegetative hyphae hyaline to fuliginous, (2—)3-4(-6) um

wide. CONIDIOGENOUS CELLS monoblastic, intercalary, determinate, slightly

denticulate or thickened, scarred, hyaline. Conidial secession schizolytic.

ConipiA, solitary, hyaline, branched; mature conidia comprise a short main

axis [(15-)16-20(-32) x 4-5 um, (0-)1-2(-3)-septate] and (almost always)

four straight branches [(10-)14-38(-42) x 3-5 um, (1-)2(-6)-septate]; three

of the branches are produced from the terminal cell of the main axis (a lateral

branch, a distal branch, and a sharply reflexed branch that runs parallel to

the main axis); the fourth branch is lateral and produced from the proximal

cell of the reflexed branch; occasional conidia have either more or fewer than

4 branches.

Tripospermum sinense sp. nov. (China) ... 515

PLATE 1. Tripospermum sinense (holotype, YMF 1.04181).

A. Conidia; B. Conidiogenous cells and conidia.

Scale bars = 10 um.

516 ... Qiao, Huang & al.

Discussion

Tripospermum sinense fits well within the genus based on its conidiogenous

cells, developmental process, branching pattern, and conidial shape. It resembles

three species: T. camelopardus Ingold et al., T. myrti (Lind) S. Hughes, and

T. porosporiferum Matsush. Tripospermum camelopardus is distinguished by

conidia that generally have three lateral branches, with only one lateral branch

attached to its main axis (Ingold et al. 1968). Like T. sinense, T. myrti produces

5-radiate conidia but differs by its smaller conidia [main axis 5-12 x 2.7 um;

parallel branches 13-30 x 4-5 um with the other branches <35 um long] and

phialidic conidiogenous cells (Hughes 1951, Ingold & Cox 1957, Rocha et al.

2010). Tripospermum porosporiferum produces similarly shaped conidia, but its

conidia are larger (Matsushima 1993), and its hyphae are about twice as wide as

those of T. sinense (Rocha et al. 2010).

Tripospermum is morphologically similar to Retiarius D.L. Olivier. The

mature conidia of the type species, R. superficiaris D.L. Olivier, resemble those

of T. sinense, but conidia development substantially differs in R. superficiaris:

the conidia branch dichotomously in early stages of development, then one

of the two lobes branches dichotomously to produce a downwardly directed

branch and a radially directed branch (Olivier 1978). In T. sinense, the conidial

main axis produces thee branches from its distal cell, one of which is sharply

reflexed to run parallel to the main axis, and a fourth branch is produced from

the proximal cell of the reflexed branch.

Also, Tripospermum sinense (growing on dead submerged leaves) has a very

different habitat from the two described Retiarius species (parasitic on pollen

grains on the phylloplane of aerial living leaves; Olivier 1978). Therefore our

new species has affinities with Tripospermum, rather than Retiarius.

Acknowledgements

This work was jointly financed by National Natural Science Foundation Program

of PR China (31360130, 31570023). We are very grateful to Prof. X.G. Zhang and

Dr. R.E. Castafeda-Ruiz for critically reviewing the manuscript and providing helpful

suggestions to improve this paper.

Literature cited

Bai YL, Li JY, Qiao M, Qian WY, Yang GZ, Yu ZF. 2013. Setosynnema yunnanense sp. nov. from

submerged decaying leaves. Mycotaxon 125: 81-85. https://doi.org/10.5248/125.81

Ellis MB. 1971. Dematiaceous hyphomycetes, CABI, Kew Surrey, England. 608 p.

Guo MT, Qiao M, Li JY, Wang W, Yu ZE 2015. Verticicladus hainanensis, a new aquatic hyphomycete.

Mycotaxon 130: 275-278. https://doi.org/10.5248/130.275

Tripospermum sinense sp. nov. (China) ... 517

Hughes SJ. 1951. Studies on micro-fungi XII. Triposporium, Tripospermum, Ceratosporella, and

Tetraposporium (gen. nov.). Mycological Papers 46. 35 p.

Index Fungorum 2016. http://www.indexfungorum.org/names/Names.asp [accessed 27.11.2016].

Ingold CT, Cox VJ. 1957. On Tripospermum and Campylospora. Transactions of the British

Mycological Society 40: 317-321. https://doi.org/10.1016/S0007-1536(57)80027-8

Ingold CT, Dann V, McDougall PJ. 1968. Tripospermum camelopardus sp. nov. Transactions of the

British Mycological Society 51: 51-56. https://doi.org/10.1016/S0007-1536(68)80121-4

Li JY, Qiao M, Peng J, Qian WY, Yang GZ, Yu ZF. 2014. Uncispora hainanensis sp. nov. isolated from

decayed leaves. Mycotaxon 129: 473-476. https://doi.org/10.5248/129.473

Ma J, Wang Y, Ma LG, Zhang YD, Castafieda-Ruiz RF, Zhang XG. 201lla. Three new

species of Neosporidesmium from Hainan, China. Mycological Progress 10: 157-162.

https://doi.org/10.1007/s11557-010-0685-2

Ma J, Wang Y, O’Neill NR, Zhang XG. 2011b. A revision of the genus Lomaantha, with the

description of a new species. Mycologia 103: 407-410. https://doi.org/10.3852/10-176

Matsushima T. 1980. Saprophytic microfungi from Taiwan. Part 1. Hyphomycetes. Matsushima

Mycological Memoirs No, 1, 1-82.

Matsushima T. 1983. Matsushima Mycological Memoirs No. 3. Matsushima Fungus Collection,

Kobe, Japan.

Matsushima T. 1993. Matsushima Mycological Memoirs No.7. Published by the Author, Kobe,

Japan.

Olivier DL. 1978. Retiarius gen. nov.: phyllosphere fungi which capture wind-borne pollen

grains. Transactions of the British Mycological Society 71: 193-201.

https://doi.org/10.1016/S0007-1536(78)80098-9

Ren SC, Ma J, Ma LG, Zhang YD, Zhang XG. 2012. Sativumoides and Cladosporiopsis,

two new genera of hyphomycetes from China. Mycological Progress 11: 443-448.

https://doi.org/10.1007/s11557-011-0759-9

Rocha FB, Barreto RW, Bezerra JL, Neto, JAAM. 2010. Foliar mycobiota of Coussapoa floccosa,

a highly threatened tree of the Brazilian Atlantic Forest. Mycologia 102: 1240-1252.

https://doi.org/10.3852/09-178

Spegazzini C. 1918. Notas micoldgicas. Physis 4: 281-295.

Yang GZ, Lu J, Yu ZE, Zhang K, Qiao M. 2011. Uncispora sinense, a new species from China.

Mycotaxon 116: 171-174. https://doi.org/10.5248/116.171

Yang GZ, Lu KP, Yang Y, Ma LB, Qiao M, Zhang KQ, Yu ZE. 2012. Sympodioplanus yunnanensis,

a new aquatic species. Mycotaxon 120: 287-290. https://doi.org/10.5248/120.287

Zhang K, Ma LG, Zhang XG. 2009. New species and records of Shrungabeeja from southern China.

Mycologia 101: 573-578. https://doi.org 10.3852/09-006

MY COTAXON

July-September 2017— Volume 132, pp. 519-523

https://doi.org/10.5248/132.519

Calceispora hachijoensis and Paratomenticola lanceolata,

newly recorded from China

X1A0-MEI WANG", Guo-NinG Liv’, X1A0-MAN Liu’ & X1A0-FENG Du’

‘College of Agronomy, Jilin Agricultural University, Changchun, 130118, China

*Baicheng Normal University, Baicheng, 137000, China

*CORRESPONDENCE TO: wxm820@126.com; communicate2007@126.com

ABSTRACT—Calceispora hachijoensis and Paratomenticola lanceolata are described and

illustrated, based on Chinese collections on decaying twigs of unidentified broadleaf plants.

These species are new records for China.

KEY worDs—anamorphic fungi, taxonomy

Introduction

Several studies on conidial fungi developed in southern China have revealed

a high number of novel taxa and new records (e.g., Guo et al. 2015; Ma 2016;

Ma et al. 2016; Xia et al. 2015a,b, 2016a,b). During a mycological survey of

conidial fungi occurring on decaying plant material from Henan and Guangxi

provinces, China, specimens of Calceispora hachijoensis and Paratomenticola

lanceolata were collected on decaying twigs. The specimens are described

and illustrated below and are deposited in the Herbarium of Mycology, Jilin

Agricultural University, Changchun, China (HMJAU).

Calceispora hachijoensis Matsush., Icon. Microfung. Matsush. Lect.: 20. 1975. Fic. 1

CONIDIOPHORES distinct, solitary, unbranched, cylindrical, 110-320 x 5-7

um, <12-septate, brown to dark brown, paler toward the apex. CONIDIOGENOUS

CELLS monophialidic, terminal, integrated, cylindrical, slightly swollen toward

the belly, <6.5 um diam., producing conidia by percurrent non-progressive

proliferation from a single fertile locus at the apex where there is a prominent,

520 ... Wang & al.

Fic. 1. Calceispora hachijoensis (HMJAU H7859). A. Conidiophores, conidiogenous cells, and

conidia; B. Conidiophores and conidiogenous cells; C. Conidia; D. Conidiogenous cells and

conidia.

New Calceispora & Paratomenticola records for China... 521

slightly narrower collarette, 3.5-4.5 um wide. Conip14 colourless, subglobose to

oval, thick-walled, aseptate, 13.5-18.5 x 10.5-12 um, with a single, unbranched

appendage at each end.

SPECIMEN EXAMINED: CHINA, GUANGXI PROVINCE, Longhu Mountain, on decaying

twigs of an unidentified broadleaf tree, 10 Dec. 2015, C.L. Yang (HMJAU H7859).

CoMMENTS—Calceispora Matsush. was erected with C. hachijoensis as type

species (Matsushima 1975) and remained monotypic until Sutton (1993)

described C. subglobosa B. Sutton. Calceispora is characterized by monophialidic

conidiogenous cells that produce hyaline aseptate conidia with unbranched

cellular appendages. Our specimen fits well with the original description of

C. hachijoensis (Matsushima 1975). This is the first report of this species in

China.

Paratomenticola lanceolata (Cooke) M.B. Ellis,

More Demat. Hyphomyc.: 175. 1976. FIG. 2

CONIDIOPHORES macronematous, mononematous, erect, usually fasciculate

(occasionally single), flexuous, smooth, septate, brown to dark reddish brown,

sometimes paler toward the base, 70-110 x 8-10 um. CONIDIOGENOUS CELLS

polyblastic, sympodial, integrated, terminal becoming intercalary, cylindrical,

denticulate, brown to dark reddish brown, 15-20 x 7-9 um, conicotruncate at

the apex. ConipiA solitary, obclavate, straight or slightly flexuous, pale brown

to brown, paler toward the apex, 7-13-distoseptate, 50-95 x 7.5-10.5 um,

tapering gradually to 2.5-4 um diam. near the apex, with a dark brown to black,

2.5-3.5 um wide scar at the base.

SPECIMEN EXAMINED: CHINA, HENAN PROVINCE, Jingangtai National Forest Park,

on decaying twigs of an unidentified broadleaf tree, 4 Nov. 2015, C.L. Yang (HMJAU

H7844).

CoMMENTS—Paratomenticola M.B. Ellis was erected with Helminthosporium

lanceolatum Cooke [= Paratomenticola lanceolata] as type species (Ellis

1976), and remained monotypic until J.L. Crane & Schokn. (1982) described

P. georgiana. Our specimen is similar to previous descriptions of the type

specimen of P lanceolata, except that the type had fewer (5-9) conidial septa

(Cooke 1883, as “H. lanceolatum”; Ellis 1976). Paratomenticola lanceolata is

reported for the first time from China.

Acknowledgments

The authors express gratitude to Dr. Xiu-Guo Zhang and Dr. Jian Ma for serving as

pre-submission reviewers and for their valuable comments and suggestions. This project

was supported by the National Natural Science Foundation of China (30600003). We

522 ... Wang & al.

20 wm

Fic. 2. Paratomenticola lanceolata (HMJAU H7844). A. Conidiophores, conidiogenous cells, and

conidia; B. Conidiophores and conidiogenous cells; C. Conidia.

New Calceispora & Paratomenticola records for China ... 523

acknowledge the facilities provided by Dr. P.M. Kirk and Drs. V. Robert, G. Stegehuis,

and Arthur Decock through the Index Fungorum and MycoBank websites. Dr. Lorelei

Norvell’s editorial review and Dr. Shaun Pennycook’s nomenclature review are greatly

appreciated.

Literature cited

Cooke MC. 1883. New American fungi. Grevillea 12(61): 22-33.

Crane JL, Schoknecht JD. 1982. Hyphomycetes from freshwater swamps and hammocks. Canadian

Journal of Botany 60(4): 369-378. https://doi.org/10.1139/b82-051

Ellis MB. 1976. More dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew,

Surrey, England.

Guo MT, Qiao M, Li JY, Wang W, Yu ZE 2015. Verticicladus hainanensis, a new aquatic hyphomycete.

Mycotaxon 130(1): 275-278. https://doi.org/10.5248/130.275

Ma J. 2016. Corynesporopsis obclavata and Stanjehughesia jiangxiensis spp. nov. from Lushan

Mountain, China. Mycotaxon 131(3): 583-588. http://dx.doi.org/10.5248/131.583

Ma J, Zhang K, Zhang KXG, Castafieda-Ruiz RF. 2016. Three new species of Spadicoides from

Lushan Mountain, China. Mycological Progress 15: 43 [8 p.].

https://doi.org/10.1007/s11557-016-1185-9

Matsushima T. 1975. Icones microfungorum a Matsushima lectorum. Published by the Author,

Kobe, Japan.

Sutton BC. 1993. Mitosporic fungi from Malawi. Mycological Papers 167. 93 p.

Xia JW, Ma YR, Gao JM, Li Z, Zhang XG. 2015a. Sporidesmiopsis malloti sp. nov. and new records

from southern China. Mycotaxon 130(3): 827-833. https://doi.org/10.5248/130.827

Xia JW, Ma YR, Gao JM, Li Z, Zhang XG. 2015b. Codinaea jianfenglingensis sp. nov. and new

records from southern China. Mycotaxon 130(3): 835-841. https://doi.org/10.5248/130.835

Xia JW, Ma YR, Gao JM, Li Z, Zhang XG. 2016a. Sympodiosynnema, a new genus of dematiaceous

hyphomycetes from southern China. Mycotaxon 131(1): 45-48. https://doi.org/10.5248/131.45

Xia JW, Ma YR, Gao JM, Zhang XG, Li Z. 2016b. Two new species of Endophragmiella from southern

China. Nova Hedwigia 103: 349-357. https://doi.org/10.1127/nova_hedwigia/2016/0356

MY COTAXON

July-September 2017— Volume 132, pp. 525-529

https://doi.org/10.5248/132.525

Records of Beltrania rhombica and

Didymobotryum rigidum from China

X1A0-MEI WANG’, Guo-NING Liv’, SHAN-SHAN CHEN’ & XIAO-FENG Du’

College of Agronomy, Jilin Agricultural University, Changchun, 130118, China

? Baicheng Normal University, Baicheng, 137000, China

* CORRESPONDENCE TO: wxm820@126.com; communicate2007@126.com

ABSTRACT—Beltrania rhombica and Didymobotryum rigidum are described and illustrated,

based on Chinese collections on decaying twigs of unidentified broadleaf plants. Beltrania

rhombica represents a new record for mainland China, and D. rigidum a new record for

Guangdong Province.

KEY worDs—anamorphic fungi, taxonomy

Introduction

Beltrania Penz. is typified by B. rhombica, which was described from Citrus

limonum |= Citrus limon] in Italy (Penzig 1882). This genus was described

with distinguishing characters such as setae with radially lobed basal cells,

conidiophores with separating cells, and biconic conidia with a hyaline

transverse band and apical tubular appendage. Didymobotryum Sacc., which

was established with Periconia rigida Berk. & Broome [= D. rigidum] as the

type species, was mainly characterized by synnematous conidiophores and

monotretic conidiogenous cells that produce unbranched acropetal chains of

1-septate conidia (Saccardo 1886).

Tropical and subtropical forest ecosystems offer particularly suitable habitats

for saprobic microfungi, among which the anamorphic are the most abundant

and diverse (e.g., Ma 2016; Ma et al. 2016; Xia et al. 2015a,b, 2016a,b). During a

mycological survey of conidial fungi occurring on decaying plant material from

Henan and Guangdong provinces, China, specimens of Beltrania rhombica and

526 ...Wang & al.

Didymobotryum rigidum were collected on decaying twigs. The specimens

are described and illustrated below and are deposited in the Herbarium of

Mycology, Jilin Agricultural University, Changchun, China (HMJAU).

Beltrania rhombica Penz., Michelia 2(8): 474. 1882. FIG. 1

CONIDIOPHORES macronematous, mononematous, arising in groups from

the terminal cells of the immersed mycelium, erect, ascending, straight or

20 um

Fic. 1. Beltrania rhombica (HMJAU H6692). Conidiophores, conidiogenous cells, and conidia.

New Beltrania & Didymobotryum records for China ... 527

flexuous, unbranched, thick-walled, 4-8-septate, pale brown to mid brown,

geniculate, conidial scars visible after secession of the conidia, 90-170 x 3-5

um. CONIDIOGENOUS CELLS polyblastic, integrated, terminal, becoming lateral,

determinate, cylindrical. Conip1a solitary, dry, acrogenous or acropleurogenus,

simple, biconic, smooth, slightly truncate at the base, with a rostrum at the

apex, subhyaline to pale brown, with a hyaline median pale transverse band,

22-28 x 7.5-10 um (rostrum exclude), rostrum 4.5-7 um long.

SPECIMEN EXAMINED: CHINA, HENAN PROVINCE, Jigong Mountain, on decaying twigs

of an unidentified broadleaf tree, 2 Nov. 2016, J.W. Xia (HMJAU H6692).

ComMENTS—Beltrania rhombica is easily recognizable by its biconic conidia

with a rostrum. Our specimen fits well with the original description of B.

rhombica by Penzig (1882) except for the lack of setae. This species is reported

for the first time from mainland China.

Didymobotryum rigidum (Berk. & Broome) Sacc., Syll. Fung. 4: 627. 1886. Fie. 2

CONIDIOMATA synnematal, unbranched, erect, with brown to dark brown

stalks, consisting of compact aggregation of parallel conidiophores, terminating

in brown fertile heads, <1000 um long and 35-45 um diam. in the middle,

<110 um diam. at the base, and 260 um diam. at the apex. CONIDIOPHORES

macronematous, erect, unbranched, septate, pale brown to brown, 2-4

um diam. CONIDIOGENOUS CELLS monotretic, integrated, terminal, thick-

walled, smooth, brown. Conidial secession schizolytic. Conip1a solitary, dry,

ellipsoidal, 1-euseptate, smooth, brown, 11-12.5 x 4.5-6 um; septa 1.5-2.5 um

thick, darkly pigmented.

SPECIMEN EXAMINED: CHINA, GUANGDONG PROVINCE, Baiyun Mountain, on

decaying twigs of an unidentified broadleaf tree, 6 Jun. 2015, J.W. Xia (HMJAU H6383).

CoMMENTS—Didymobotryum rigidum is easily recognizable by its

synnematous conidiophores and monotretic conidiogenous cells that produce

catenate ellipsoidal conidia. This species has previously been reported from Sri

Lanka, Thailand, Vietnam, China (Hong Kong), Japan, and Cuba (Berkeley

& Broome 1873; Ellis 1971; Jones et al. 2004; Kobayashi 2007; Lu et al. 2000;

Mercado Sierra et al. 1997; Minter et al. 2001; Saccardo 1886; Zhuang 2001);

it is reported here for the first time from Guangdong Province.

Acknowledgments

The authors express gratitude to Dr. Xiu-Guo Zhang and Dr. Jian Ma for serving as

pre-submission reviewers and for their valuable comments and suggestions. This project

was supported by the National Natural Science Foundation of China (30600003). We

acknowledge the facilities provided by Dr. P.M. Kirk and Drs. V. Robert, G. Stegehuis

528 ...Wang & al.

Fic. 2. Didymobotryum rigidum (HMJAU H6383).

A. Synnema; B. Conidia; C-E. Conidiophores, conidiogenous cells, and conidia.

New Beltrania & Didymobotryum records for China ... 529

and Arthur Decock through the Index Fungorum and MycoBank websites. Dr. Lorelei

Norvell’s editorial review and Dr. Shaun Pennycook’s nomenclature review are greatly

appreciated.

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Mountain, China. Mycotaxon 131(3): 583-588. https://doi.org/10.5248/131.583

Ma J, Zhang K, Zhang XG, Castafeda-Ruiz RF. 2016. Three new species of Spadicoides from Lushan

Mountain, China. Mycological Progress 15: 43 [8 p.]. https://doi.org/10.1007/s11557-016-1185-9

Mercado Sierra A, Holubova-Jechova V, Mena Portales J. 1997. Hifomicetes demaciaceos de Cuba:

enteroblasticos. Museo Regionale di Scienze Naturali, Torino, Monografie 23. 388 p.

Minter DW, Rodriguez Hernandez M, Mena Portales J. 2001. Fungi of the Caribbean: an annotated

checklist. PDMS Publishing Isleworth, UK. 946 p.

Penzig O. 1882. Funghi agrumicoli: contribuzione allo studio dei funghi parassiti degli agrumi.

Michelia 2(8): 385-508.

Saccardo PA. 1886. Sylloge Hyphomycetum. Sylloge Fungorum 4. 807 p.